Molecular epidemiology of Kaposi sarcoma virus in Spain

被引:4
作者
Gomez, Inmaculada P. [1 ]
Perez-Vazquez, Maria Dolores P. [1 ]
Tarrago, David P. [1 ,2 ]
机构
[1] Inst Salud Carlos III, Ctr Nacl Microbiol CNM, Majadahonda, Spain
[2] CIBER Epidemiol & Publ Hlth CIBERESP, Madrid, Spain
关键词
HERPESVIRUS KSHV; NORTH-AMERICA; AIDS; AFRICA; HUMAN-HERPESVIRUS-8; GENOTYPES; VARIANTS; SUBTYPE; DIVERSITY; DISEASE;
D O I
10.1371/journal.pone.0274058
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
BackgroundSince human herpesvirus 8 (HHV-8) infection may be underestimated and HHV-8 subtype circulation in Spain remains unknown, a molecular epidemiologic study is highly desirable. ObjectivesThis study aimed to analyse HHV-8 subtype diversity and their distribution in Spain. Study designThe study included 142 HHV-8 infected patients. A nested PCR was developed in order to permit Sanger sequencing of HHV-8 K1 ORF directly from clinical samples received at the CNM from 2013 to 2021. Phylogenetic characterization was performed. ResultsGenotypes A and C comprised 55.6% and 42.3% of strains. Regarding subtypes, 25.4% of strains were C3, 19.7% were A3, 14.1% were A5, and C2, A1, A4, C1, A2, C7 were 11.3%, 11.3%, 8.5%, 4.2%, 2.1% and 1.4%, respectively. Subtype E1, E2 and B1 were found in only one patient each (0.7%). The Madrid region accounted for 52.1% of patients and showed a significantly different subtype distribution compared to the others (P = 0.018). Subtypes B1, E1, and E2 were observed to appear sporadically, although overall genotypes A and subtype C3 remained the most frequent and unwavering. Subtype A3 presented the highest diversity as displayed by the highest number of clusters in phylogenetic analysis. Non-significant differences in viral loads between genotypes were found, but significantly higher viral loads in subtype C2 compared to subtype C3 was found, while no significant subtype differences were observed between subtypes within genotype A. Infections with HHV-8 were detected in 94 (66.2%) patients without KS and compared to patients with KS non-significant differences in subtype distribution were found. ConclusionsSubtype prevalence and regional distribution followed a similar pattern compared to other western European countries. Our study is the first to report HHV-8 subtypes E1 and E2 circulating in Europe that might be reflective of migration of population from Caribbean countries. Our study suggests that infection by HHV-8 is underestimated, and wider screening should be recommended for risk groups.
引用
收藏
页数:13
相关论文
共 35 条
[1]  
Boralevi F, 1996, Rev Prat, V46, P1952
[2]   IDENTIFICATION OF HERPESVIRUS-LIKE DNA-SEQUENCES IN AIDS-ASSOCIATED KAPOSIS-SARCOMA [J].
CHANG, Y ;
CESARMAN, E ;
PESSIN, MS ;
LEE, F ;
CULPEPPER, J ;
KNOWLES, DM ;
MOORE, PS .
SCIENCE, 1994, 266 (5192) :1865-1869
[3]   Variability and evolution of Kaposi's sarcoma-associated herpesvirus in Europe and Africa [J].
Cook, PM ;
Whitby, D ;
Calabro, ML ;
Luppi, M ;
Kakoola, DN ;
Hjalgrim, H ;
Ariyoshi, K ;
Ensoli, B ;
Davison, AJ ;
Schulz, TF .
AIDS, 1999, 13 (10) :1165-1176
[4]   Analysis of the ORFK1 hypervariable regions reveal distinct HHV-8 clustering in Kaposi's sarcoma and non-Kaposi's cases [J].
Cordiali-Fei, Paola ;
Trento, Elisabetta ;
Giovanetti, Marta ;
Lo Presti, Alessandra ;
Latini, Alessandra ;
Giuliani, Massimo ;
D'Agosto, Giovanna ;
Bordignon, Valentina ;
Cella, Eleonora ;
Farchi, Francesca ;
Ferraro, Carmela ;
La Parola, Ilaria Lesnoni ;
Cota, Carlo ;
Sperduti, Isabella ;
Vento, Antonella ;
Cristaudo, Antonio ;
Ciccozzi, Massimo ;
Ensoli, Fabrizio .
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH, 2015, 34
[5]  
Fouchard N, 2000, INT J CANCER, V85, P166, DOI 10.1002/(SICI)1097-0215(20000115)85:2<166::AID-IJC3>3.0.CO
[6]  
2-
[7]   KSHV strains: the origins and global spread of the virus [J].
Hayward, GS .
SEMINARS IN CANCER BIOLOGY, 1999, 9 (03) :187-199
[8]   Imported cases of malaria in Spain: observational study using nationally reported statistics and surveillance data, 2002-2015 [J].
Herrador, Zaida ;
Fernandez-Martinez, Beatriz ;
Quesada-Cubo, Victor ;
Diaz-Garcia, Oliva ;
Cano, Rosa ;
Benito, Agustin ;
Gomez-Barroso, Diana .
MALARIA JOURNAL, 2019, 18 (1)
[9]   Genetic Diversity of HHV8 Subtypes in South Africa: A5 Subtype Is Associated With Extensive Disease in AIDS-KS [J].
Isaacs, Thuraya ;
Abera, Aron B. ;
Muloiwa, Rudzani ;
Katz, Arieh A. ;
Todd, Gail .
JOURNAL OF MEDICAL VIROLOGY, 2016, 88 (02) :292-303
[10]   New Kaposi's sarcoma-associated herpesvirus variant in men who have sex with men associated with severe pathologies [J].
Jary, Aude ;
Leducq, Valentin ;
Desire, Nathalie ;
Petit, Heloise ;
Palich, Romain ;
Joly, Veronique ;
Canestri, Ana ;
Gothland, Adelie ;
Lambert-Niclot, Sidonie ;
Surgers, Laure ;
Amiel, Corinne ;
Descamps, Diane ;
Spano, Jean-Philippe ;
Katlama, Christine ;
Calvez, Vincent ;
Marcelin, Anne-Genevieve .
JOURNAL OF INFECTIOUS DISEASES, 2020, 222 (08) :1320-1328