Magnetic nanoparticle decorated graphene based electrochemical nanobiosensor for H2O2 sensing using HRP

被引：37
|
作者：
Waifalkar, P. P. [1 ]
Chougale, A. D. [2 ]
Kollu, P. [3 ,4 ]
Patil, P. S. [1 ]
Patil, P. B. [5 ]
机构：
[1] Shivaji Univ, Dept Phys, Thin Film Mat Lab, Kolhapur, MS, India
[2] Shivaji Univ, New Coll, Dept Chem, Kolhapur, MS, India
[3] Indian Inst Technol, Dept Met Engn & Mat Sci, Mumbai, MS, India
[4] Univ Cambridge, Dept Phys, Cavendish Lab, Thin Film Magnetism Grp, Cambridge CB3 0HE, England
[5] Shivaji Univ, New Coll, Dept Phys, Kolhapur, MS, India
来源：
COLLOIDS AND SURFACES B-BIOINTERFACES | 2018年 / 167卷
关键词：
Magnetic nanoparticle decorated graphene; MNP; RGO; H2O2; Horseradish peroxidase; Electrochemical biosensor; AMPEROMETRIC HYDROGEN-PEROXIDE; HORSERADISH-PEROXIDASE; FUNCTIONALIZED-GRAPHENE; CARBON NANOTUBES; COMPOSITE FILM; BIOSENSOR; IMMOBILIZATION; CHITOSAN; ELECTRODE; SENSOR;
D O I：
10.1016/j.colsurfb.2018.04.042
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
To utilize synergetic effect of graphene's higher conductivity and magnetic nanoparticles biocompatibility, an electrochemical nanobiosensor is constructed based on magnetic nanoparticle decorated graphene (MRGO) using Horseradish peroxidase (HRP) for H2O2 sensing. Sensors based on magnetic nanoparticles (MNP) and reduced graphene oxide (RGO) are studied for comparison. MNP, RGO and MRGO were characterized by X-ray diffraction (XRD), Transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy (FTIR). XRD studies have confirmed successful synthesis of Fe3O4 MNPs, RGO and MRGO. TEM micrographs revealed uniform decoration of MNPs on graphene. FTIR confirmed the immobilization of HRP on MNP, RGO and MRGO. The MRGO based sensor exhibited higher sensitivity (48.08 mu A mu M-1 cm(-2)) compared to MNP (39.08 mu A mu M-1 cm(-2)) and RGO (41.08 mu A mu M-1 cm(-2)) based biosensors. (C) 2018 Elsevier B.V. All rights reserved.
引用
收藏
页码：425 / 431
页数：7
相关论文
共 50 条
  • [21] Graphene enhanced electrochemiluminescence of CdS nanocrystal for H2O2 sensing
    Wang, Kun
    Liu, Qian
    Wu, Xiang-Yang
    Guan, Qing-Meng
    Li, He-Nan
    TALANTA, 2010, 82 (01) : 372 - 376
  • [22] Hydrothermal Fabricated Ag Nanoparticles-decorated Reduced Graphene Oxide Composite for H2O2 Electrochemical Detection
    Wu, Shuisheng
    Tan, Nianyuan
    Lan, Donghui
    Au, Chak-Tong
    Yi, Bing
    INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE, 2020, 15 (07): : 6155 - 6164
  • [23] The application of the QDs/H2O2 chemiluminescence system in HRP assay and HRP-based immunoassay
    Ghavamipour, Fahimeh
    Khajeh, Khosro
    Sajedi, Reza H.
    COLLOIDS AND SURFACES B-BIOINTERFACES, 2021, 206
  • [24] Efficient Amperometric Detection of H2O2 using Gold Nanoparticle decorated Polythiophene/Hematite Ore Nanocomposite
    Rashed, Md. A.
    Rahman, M. Hafizur
    Nayem, N. I.
    Ahmed, Jahir
    Faisal, M.
    Jalalah, Mohammed
    Harraz, Farid A.
    JOURNAL OF THE ELECTROCHEMICAL SOCIETY, 2024, 171 (02)
  • [25] Development of HRP-modified Carbon Composite Biosensor and Electrochemical Analysis of H2O2
    Park, Deog-Su
    JOURNAL OF THE KOREAN CHEMICAL SOCIETY-DAEHAN HWAHAK HOE JEE, 2012, 56 (05): : 571 - 576
  • [26] Electrochemical sensing of H2O2 using cobalt oxide modified TiO2 nanotubes
    Ullah, Rahim
    Rasheed, Muhammad Asim
    Abbas, Shafqat
    Rehman, Khalil-ul
    Shah, Attaullah
    Ullah, Karim
    Khan, Yaqoob
    Bibi, Maryam
    Ahmad, Mashkoor
    Ali, Ghafar
    CURRENT APPLIED PHYSICS, 2022, 38 : 40 - 48
  • [27] Electrochemical H2O2 biosensor composed of myoglobin on MoS2 nanoparticle-graphene oxide hybrid structure
    Yoon, Jinho
    Lee, Taek
    Bapurao, Bharate G.
    Jo, Jinhee
    Oh, Byung-Keun
    Choi, Jeong-Woo
    BIOSENSORS & BIOELECTRONICS, 2017, 93 : 14 - 20
  • [28] Hemoglobin-graphene modified carbon fiber microelectrode for direct electrochemistry and electrochemical H2O2 sensing
    Bai, Jie
    Wu, Liping
    Wang, Xiaojuan
    Zhang, Hui-Min
    ELECTROCHIMICA ACTA, 2015, 185 : 142 - 147
  • [29] H2O2 sensing using HRP modified catalyst-free ZnO nanorods synthesized by RF sputtering
    Amit Srivastava
    Naresh Kumar
    Priti Singh
    Sunil Kumar Singh
    Applied Physics A, 2017, 123
  • [30] H2O2 sensing using HRP modified catalyst-free ZnO nanorods synthesized by RF sputtering
    Srivastava, Amit
    Kumar, Naresh
    Singh, Priti
    Singh, Sunil Kumar
    APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING, 2017, 123 (06):
12345