Magnetic nanoparticle decorated graphene based electrochemical nanobiosensor for H2O2 sensing using HRP

被引：37
|
作者：
Waifalkar, P. P. [1 ]
Chougale, A. D. [2 ]
Kollu, P. [3 ,4 ]
Patil, P. S. [1 ]
Patil, P. B. [5 ]
机构：
[1] Shivaji Univ, Dept Phys, Thin Film Mat Lab, Kolhapur, MS, India
[2] Shivaji Univ, New Coll, Dept Chem, Kolhapur, MS, India
[3] Indian Inst Technol, Dept Met Engn & Mat Sci, Mumbai, MS, India
[4] Univ Cambridge, Dept Phys, Cavendish Lab, Thin Film Magnetism Grp, Cambridge CB3 0HE, England
[5] Shivaji Univ, New Coll, Dept Phys, Kolhapur, MS, India
来源：
COLLOIDS AND SURFACES B-BIOINTERFACES | 2018年 / 167卷
关键词：
Magnetic nanoparticle decorated graphene; MNP; RGO; H2O2; Horseradish peroxidase; Electrochemical biosensor; AMPEROMETRIC HYDROGEN-PEROXIDE; HORSERADISH-PEROXIDASE; FUNCTIONALIZED-GRAPHENE; CARBON NANOTUBES; COMPOSITE FILM; BIOSENSOR; IMMOBILIZATION; CHITOSAN; ELECTRODE; SENSOR;
D O I：
10.1016/j.colsurfb.2018.04.042
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
To utilize synergetic effect of graphene's higher conductivity and magnetic nanoparticles biocompatibility, an electrochemical nanobiosensor is constructed based on magnetic nanoparticle decorated graphene (MRGO) using Horseradish peroxidase (HRP) for H2O2 sensing. Sensors based on magnetic nanoparticles (MNP) and reduced graphene oxide (RGO) are studied for comparison. MNP, RGO and MRGO were characterized by X-ray diffraction (XRD), Transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy (FTIR). XRD studies have confirmed successful synthesis of Fe3O4 MNPs, RGO and MRGO. TEM micrographs revealed uniform decoration of MNPs on graphene. FTIR confirmed the immobilization of HRP on MNP, RGO and MRGO. The MRGO based sensor exhibited higher sensitivity (48.08 mu A mu M-1 cm(-2)) compared to MNP (39.08 mu A mu M-1 cm(-2)) and RGO (41.08 mu A mu M-1 cm(-2)) based biosensors. (C) 2018 Elsevier B.V. All rights reserved.
引用
收藏
页码：425 / 431
页数：7
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