Extraction and identification of platelet-derived microparticles

被引：11

作者：

Guo, Jun ^{[1
]}

Feng, Can ^{[2
]}

Zhang, Bili ^{[3
]}

Zhang, Shiyang ^{[1
]}

Shen, Xiaxian ^{[3
]}

Zhu, Jiaqi ^{[3
]}

Zhao, Xian-Xian ^{[3
]}

机构：

[1] Anhui Prov Hosp, Dept Geriatr, Hefei 230000, Anhui, Peoples R China

[2] Shanghai Univ Tradit Chinese Med, Dept Cardiol, Yueyang Hosp Integrated Tradit Chinese & Western, Shanghai 200433, Peoples R China

[3] Second Mil Med Univ, Dept Cardiol, Changhai Hosp, 168 Changhai Rd, Shanghai 200433, Peoples R China

来源：

MOLECULAR MEDICINE REPORTS | 2019年 / 20卷 / 03期

基金：

中国国家自然科学基金;

关键词：

platelets; microparticles; transmission electron microscopy; flow cytometry; surface protein; EXTRACELLULAR VESICLES; HEMOSTASIS; MECHANISM; STORAGE; ORIGIN;

D O I：

10.3892/mmr.2019.10484

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Microparticles are carriers of signals for intracellular signal transduction. These carriers include proteins, mRNAs, microRNAs and other bioactive substances. Platelets are a major source of circulating microparticles, and microparticles are closely associated with the development of certain cardiovascular diseases. In the present study, a method for separating, extracting and identifying platelet-derived microparticles was developed and differences in the expression of surface proteins on microparticles harvested from platelets stimulated by vortexing or treatment with thrombin was investigated. The counts, composition, sizes and inner structures of microparticles were determined using flow cytometry and transmission electron microscopy. Additionally, it was demonstrated that platelets could be readily activated, and a large quantity of microparticles with varying complex compositions, structures and sizes were derived from activated platelets. High purity platelet-derived microparticles were obtained by gradient centrifugation. However, the microparticles derived from platelets stimulated by thrombin treatment or vortexing differed significantly in the levels of CD63. The present study aimed to provide improved options for the extraction and identification of microparticles.

引用

页码：2916 / 2921

页数：6

共 33 条

[1] Extracellular vesicles from blood plasma: determination of their morphology, size, phenotype and concentration [J].

Arraud, N. ;

Linares, R. ;

Tan, S. ;

Gounou, C. ;

Pasquet, J. -M. ;

Mornet, S. ;

Brisson, A. R. .

JOURNAL OF THROMBOSIS AND HAEMOSTASIS, 2014, 12 (05) :614-627

[2] Isolation of Exosomes from Blood Plasma: Qualitative and Quantitative Comparison of Ultracentrifugation and Size Exclusion Chromatography Methods [J].

Baranyai, Tamas ;

Herczeg, Kata ;

Onodi, Zsofia ;

Voszka, Istvan ;

Modos, Karoly ;

Marton, Nikolett ;

Nagy, Gyoergy ;

Maeger, Imre ;

Wood, Matthew J. ;

El Andaloussi, Samir ;

Palinkas, Zoltan ;

Kumar, Vikas ;

Nagy, Pater ;

Kittel, Agnes ;

Buzas, Edit Iren ;

Ferdinandy, Peter ;

Giricz, Zoltan .

PLOS ONE, 2015, 10 (12)

[3] Impaired redistribution of aminophospholipids with distinctive cell shape change during Ca2+-induced activation of platelets from a patient with Scott syndrome [J].

Bettache, N ;

Gaffet, P ;

Allegre, N ;

Maurin, L ;

Toti, F ;

Freyssinet, JM ;

Bienvenüe, A .

BRITISH JOURNAL OF HAEMATOLOGY, 1998, 101 (01) :50-58

[4] EFFECT OF COLD-STORAGE ON PLATELET GLYCOPROTEIN IB AND VESICULATION [J].

BODE, AP ;

KNUPP, CL .

TRANSFUSION, 1994, 34 (08) :690-696

[5] Influenza virus H1N1 activates platelets through FcγRIIA signaling and thrombin generation [J].

Boilard, Eric ;

Pare, Guillaume ;

Rousseau, Matthieu ;

Cloutier, Nathalie ;

Dubuc, Isabelle ;

Levesque, Tania ;

Borgeat, Pierre ;

Flamand, Louis .

BLOOD, 2014, 123 (18) :2854-2863

[6] Extracellular vesicles from activated platelets: a semiquantitative cryo-electron microscopy and immuno-gold labeling study [J].

Brisson, Alain R. ;

Tan, Sisareuth ;

Linares, Romain ;

Gounou, Celine ;

Arraud, Nicolas .

PLATELETS, 2017, 28 (03) :263-271

[7] Lipopolysaccharide Signaling without a Nucleus: Kinase Cascades Stimulate Platelet Shedding of Proinflammatory IL-1β-Rich Microparticles [J].

Brown, G. Thomas ;

McIntyre, Thomas M. .

JOURNAL OF IMMUNOLOGY, 2011, 186 (09) :5489-5496

[8]

Bulut D, 2011, CAN J PHYSIOL PHARM, V89, P239, DOI [10.1139/Y11-013, 10.1139/y11-013]

[9] Cell-derived microparticles in haemostasis and vascular medicine [J].

Burnier, Laurent ;

Fontana, Pierre ;

Kwak, Brenda R. ;

Angelillo-Scherrer, Anne .

THROMBOSIS AND HAEMOSTASIS, 2009, 101 (03) :439-451

[10] Platelet microparticles: Detection and assessment of their paradoxical functional roles in disease and regenerative medicine [J].

Burnouf, Thierry ;

Goubran, Hadi Alphonse ;

Chou, Ming-Li ;

Devos, David ;

Radosevic, Mirjana .

BLOOD REVIEWS, 2014, 28 (04) :155-166

← 1 2 3 4 →