Propolis is a substance of honeybee origin with known chemical content effects. Its chemical composition varies by geographic location, climatic zone, and local flora. In the present study, the biological and chemical contents of the propolis samples from two different regions of Denizli were determined. This study aims to analyze the Servergazi and Saraykoy propolis contents and their use for therapeutic and pharmacologic purposes. Propolis is a resinous substance collected by honey bees (Apis mellifera L.) from plants. The total phenolic content of the extracts was determined using the Folin-Ciocalteu reagents as gallic acid equivalents. The results for the propolis contents collected from the region were calculated as follows: the DPPH value of Saraykoy propolis was measured as 11.43 mg/ml, while the DPPH value of Servergazi propolis was measured as 232.778 mg/ml. As a result, the highest phenolic content was found as 223.102 mg GEA/100g in the Servergazi propolis content. According to the analysis, it was determined that the two propolis samples had rich chemical contents but the Servergazi propolis had a very rich content in all respects. In conclusion, two propolis samples of the region were very rich in phenolic substances and antioxidants; however, it was proved that the Servergazi propolis can be used for medical and pharmaceutic purposes.