Molecular characterization of wheat germplasm using microsatellite markers

被引：15

作者：

Ijaz, S. ^{[1
]}

Khan, I. A. ^{[1
]}

机构：

[1] Univ Agr Faisalabad, Ctr Agr Biochem & Biotechnol, Faisalabad, Pakistan

来源：

GENETICS AND MOLECULAR RESEARCH | 2009年 / 8卷 / 03期

关键词：

Triticum aestivum; Microsatellite; Molecular markers; Genome; Genetic distance; Population genetics; AESTIVUM L. GERMPLASM; GENETIC DIVERSITY; RICE; SEQUENCES; GENOME; RAPD;

D O I：

10.4238/vol8-3gmr608

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We investigated the genetic diversity of 63 wheat genotypes, composed of 48 accessions and 15 varieties, using 56 polymorphic simple sequence repeat primers. One hundred and eighty-six loci were found, with a mean of 131.26 alleles per locus. Cluster analysis based on microsatellite allelic diversity discriminated the accessions and varieties into different clusters; genetic diversity was the highest between variety Kohistan-97 and accession number 011512, giving a genetic similarity value of 0.4198. Accession numbers 011484 and 011356 gave a genetic similarity value of 0.9589, indicating that these accessions were 95.89% similar. We found that microsatellite markers could characterize and discriminate all of the genotypes; more primers could be used for saturation of different regions in further studies.

引用

页码：809 / 815

页数：7

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