Twelve polymorphic microsatellite loci from a dinucleotide-enriched genomic library of Japanese Spanish mackerel (Scomberomorus niphonius)

被引：7

作者：

Xing, Shi-Chao ^{[1
,2
]}

Xu, Gen-Bo ^{[1
]}

Liao, Xiao-Lin ^{[1
]}

Yang, Guan-Pin ^{[2
]}

Chen, Song-Lin ^{[1
]}

机构：

[1] Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

[2] Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China

来源：

CONSERVATION GENETICS | 2009年 / 10卷 / 04期

关键词：

Spanish mackerel; Scomberomorus niphonius; Microsatellite; Enriched genomic library; EST-LIBRARY;

D O I：

10.1007/s10592-008-9735-6

中图分类号：

X176 [生物多样性保护];

学科分类号：

090705 ;

摘要：

In the study, 34 microsatellite loci were isolated from a dinucleotide-enriched genomic library of Japanese Spanish mackerel (Scomberomorus niphonius). And 12 microsatellite loci were found to be polymorphic between 3 and 8 alleles. The number of observed and expected heterozygosity per locus in 23 individuals ranged from 0.6087 to 1.0000 and 0.8908 to 0.9773, respectively. Two loci significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction analysis and there was no significant linkage disequilibrium found between pairs of loci. As a result, 12 microsatellite loci probably located on different chromosome pairs and these polymorphic microsatellite loci should provide sufficient level of genetic diversity to investigate the fine-scale population structure and evaluate the breeding strategy in S. niphonius.

引用

页码：1167 / 1169

页数：3