A new method for the determination of Vitamin B12 in seawater

被引：57

作者：

Okbamichael, M ^{[1
]}

Sañudo-Wilhelmy, SA ^{[1
]}

机构：

[1] SUNY Stony Brook, Marine Sci Res Ctr, Stony Brook, NY 11794 USA

来源：

ANALYTICA CHIMICA ACTA | 2004年 / 517卷 / 1-2期

基金：

美国国家科学基金会;

关键词：

vitamin B-12; solid-phase extraction; seawater; HPLC;

D O I：

10.1016/j.aca.2004.05.020

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Despite the environmental importance of Vitamin B-12, there is no direct method for B-12 quantification in natural waters. Bioassay is the only protocol currently available to measure B-12 in the marine environment. Here we report a new method for the direct determination of B-12 in seawater based on C-18 solid-phase extraction and quantification by Reversed-Phase High-Performance Liquid Chromatography (HPLC) with an UV-Vis detector. The detection limit of the B-12 protocol was of the order of 0.042 ng l(-1). The method was successfully applied to the determination of Vitamin B-12 in filtered coastal seawater. Concentrations of Vitamin B-12 in the coastal environment ranged 3-605 ng l(-1) with spike recoveries between 92 and 99%. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：33 / 38

页数：6

共 36 条

[11] EFFECT OF VITAMIN CONCENTRATIONS ON GROWTH AND DEVELOPMENT OF VITAMIN-REQUIRING ALGAE [J].

CARLUCCI, AF ;

SILBERNAGEL, SB .

JOURNAL OF PHYCOLOGY, 1969, 5 (01) :64-+

[12] BIOASSAY OF SEAWATER .I. A 14C UPTAKE METHOD FOR DETERMINATION OF CONCENTRATIONS OF VITAMIN B12 IN SEAWATER [J].

CARLUCCI, AF ;

SILBERNAGEL, SB .

CANADIAN JOURNAL OF MICROBIOLOGY, 1966, 12 (01) :175-+

[13]

Chemaly SM, 2002, S AFR J SCI, V98, P568

[14] Oceans - Dis-crediting ocean fertilization [J].

Chisholm, SW ;

Falkowski, PG ;

Cullen, JJ .

SCIENCE, 2001, 294 (5541) :309-310

[15] Biogeochemical modelling of the tropical Pacific Ocean. II: Iron biogeochemistry [J].

Christian, JR ;

Verschell, MA ;

Murtugudde, R ;

Busalacchi, AJ ;

McClain, CR .

DEEP-SEA RESEARCH PART II-TOPICAL STUDIES IN OCEANOGRAPHY, 2001, 49 (1-3) :545-565

[16] VONLIEBIG LAW OF THE MINIMUM AND PLANKTON ECOLOGY (1899-1991) [J].

DEBAAR, HJW .

PROGRESS IN OCEANOGRAPHY, 1994, 33 (04) :347-386

[17] Biomarkers of genetic damage for cancer epidemiology [J].

Fenech, M .

TOXICOLOGY, 2002, 181 :411-416

[18] MINIMUM CYANOCOBALAMIN REQUIREMENTS OF SOME MARINE CENTRIC DIATOMS [J].

GUILLARD, RRL ;

CASSIE, V .

LIMNOLOGY AND OCEANOGRAPHY, 1963, 8 (02) :161-165

[19] A DETAILED ANALYSIS OF BIOLOGICALLY IMPORTANT SUBSTANCES IN A PROFILE OFF SOUTHERN CALIFORNIA [J].

HOLMHANSEN, O ;

STRICKLAND, JD ;

WILLIAMS, PM .

LIMNOLOGY AND OCEANOGRAPHY, 1966, 11 (04) :548-+

[20] Phytoplankton iron limitation in the Humboldt Current and Peru Upwelling [J].

Hutchins, DA ;

Hare, CE ;

Weaver, RS ;

Zhang, Y ;

Firme, GF ;

DiTullio, GR ;

Alm, MB ;

Riseman, SF ;

Maucher, JM ;

Geesey, ME ;

Trick, CG ;

Smith, GJ ;

Rue, EL ;

Conn, J ;

Bruland, KW .

LIMNOLOGY AND OCEANOGRAPHY, 2002, 47 (04) :997-1011

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