Precision genome engineering through adenine and cytosine base editing

被引：61
作者：
Kim, Jin-Soo [1 ,2 ]
机构：
[1] Inst for Basic Sci Korea, Ctr Genome Engn, Seoul, South Korea
[2] Seoul Natl Univ, Dept Chem, Seoul, South Korea
来源：
NATURE PLANTS | 2018年 / 4卷 / 03期
关键词：
DNA; INVERSIONS; RICE;
D O I：
10.1038/s41477-018-0115-z
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Adenine base editors (ABEs), composed of an engineered deaminase and a catalytically impaired CRISPR-Cas9 variant, are powerful new tools for targeted base editing in cells and organisms. Together with cytosine base editors (CBEs), ABEs enable single-nucleotide conversions cleanly, efficiently and reversibly without double-stranded DNA cleavage, advancing genome editing in a new dimension.
引用
收藏
页码：148 / 151
页数：4
相关论文
共 25 条
[1]   CRISPR-Mediated Base Editing Enables Efficient Disruption of Eukaryotic Genes through Induction of STOP Codons [J].
Billon, Pierre ;
Bryant, Eric E. ;
Joseph, Sarah A. ;
Nambiar, Tarun S. ;
Hayward, Samuel B. ;
Rothstein, Rodney ;
Ciccia, Alberto .
MOLECULAR CELL, 2017, 67 (06) :1068-+
[2]   Programmable base editing of A.T to G.C in genomic DNA without DNA cleavage [J].
Gaudelli, Nicole M. ;
Komor, Alexis C. ;
Rees, Holly A. ;
Packer, Michael S. ;
Badran, Ahmed H. ;
Bryson, David I. ;
Liu, David R. .
NATURE, 2017, 551 (7681) :464-+
[3]  
Hess GT, 2016, NAT METHODS, V13, P1036, DOI [10.1038/NMETH.4038, 10.1038/nmeth.4038]
[4]   Genome-wide target specificities of CRISPR RNA-guided programmable deaminases [J].
Kim, Daesik ;
Lim, Kayeong ;
Kim, Sang-Tae ;
Yoon, Sun-heui ;
Kim, Kyoungmi ;
Ryu, Seuk-Min ;
Kim, Jin-Soo .
NATURE BIOTECHNOLOGY, 2017, 35 (05) :475-+
[5]  
Kim D, 2015, NAT METHODS, V12, P237, DOI [10.1038/NMETH.3284, 10.1038/nmeth.3284]
[6]   A guide to genome engineering with programmable nucleases [J].
Kim, Hyongbum ;
Kim, Jin-Soo .
NATURE REVIEWS GENETICS, 2014, 15 (05) :321-334
[7]   Highly efficient RNA-guided base editing in mouse embryos [J].
Kim, Kyoungmi ;
Ryu, Seuk-Min ;
Kim, Sang-Tae ;
Baek, Gayoung ;
Kim, Daesik ;
Lim, Kayeong ;
Chung, Eugene ;
Kim, Sunghyun ;
Kim, Jin-Soo .
NATURE BIOTECHNOLOGY, 2017, 35 (05) :435-+
[8]   Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins [J].
Kim, Sojung ;
Kim, Daesik ;
Cho, Seung Woo ;
Kim, Jungeun ;
Kim, Jin-Soo .
GENOME RESEARCH, 2014, 24 (06) :1012-1019
[9]   Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions [J].
Kim, Y. Bill ;
Komor, Alexis C. ;
Levy, Jonathan M. ;
Packer, Michael S. ;
Zhao, Kevin T. ;
Liu, David R. .
NATURE BIOTECHNOLOGY, 2017, 35 (04) :371-+
[10]   High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects [J].
Kleinstiver, Benjamin P. ;
Pattanayak, Vikram ;
Prew, Michelle S. ;
Tsai, Shengdar Q. ;
Nguyen, Nhu T. ;
Zheng, Zongli ;
Joung, J. Keith .
NATURE, 2016, 529 (7587) :490-+
123