Dielectric Barrier Discharge Ionization in Characterization of Organic Compounds Separated on Thin-Layer Chromatography Plates

被引：16

作者：

Ceglowski, Michal ^{[1
]}

Smoluch, Marek ^{[2
]}

Babij, Michal ^{[3
]}

Gotszalk, Teodor ^{[3
]}

Silberring, Jerzy ^{[2
,4
]}

Schroeder, Grzegorz ^{[1
]}

机构：

[1] Adam Mickiewicz Univ, Fac Chem, Dept Supramol Chem, PL-60780 Poznan, Poland

[2] AGH Univ Sci & Technol, Fac Mat Sci & Ceram, Dept Biochem & Neurobiol, Krakow, Poland

[3] Wroclaw Univ Technol, Fac Microsyst Elect & Photon, PL-50370 Wroclaw, Poland

[4] Polish Acad Sci, Ctr Polymer & Carbon Mat, Zabrze, Poland

来源：

PLOS ONE | 2014年 / 9卷 / 08期

关键词：

DESORPTION/IONIZATION MASS-SPECTROMETRY; ASSISTED-LASER-DESORPTION/IONIZATION; ELECTROSPRAY-IONIZATION; DESORPTION IONIZATION; SURFACE; PROBE; TLC; MS;

D O I：

10.1371/journal.pone.0106088

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A new method for on-spot detection and characterization of organic compounds resolved on thin layer chromatography (TLC) plates has been proposed. This method combines TLC with dielectric barrier discharge ionization (DBDI), which produces stable low-temperature plasma. At first, the compounds were separated on TLC plates and then their mass spectra were directly obtained with no additional sample preparation. To obtain good quality spectra the center of a particular TLC spot was heated from the bottom to increase volatility of the compound. MS/MS analyses were also performed to additionally characterize all analytes. The detection limit of proposed method was estimated to be 100 ng/spot of compound.

引用

页数：5

共 35 条

[1]

Babij M, 2013, MINIATURE PLASMA JET

[2] MASS-SPECTROMETRIC DETECTORS FOR SAMPLES SEPARATED BY PLANAR ELECTROPHORESIS [J].

BUSCH, KL .

JOURNAL OF CHROMATOGRAPHY A, 1995, 692 (1-2) :275-290

[3] On-line overpressure thin-layer chromatographic separation and electrospray mass spectrometric detection of glycolipids [J].

Chai, WG ;

Leteux, C ;

Lawson, AM ;

Stoll, MS .

ANALYTICAL CHEMISTRY, 2003, 75 (01) :118-125

[4] DIRECT ANALYSIS OF THIN-LAYER CHROMATOGRAPHY SPOTS BY FAST ATOM BOMBARDMENT MASS-SPECTROMETRY [J].

CHANG, TT ;

LAY, JO ;

FRANCEL, RJ .

ANALYTICAL CHEMISTRY, 1984, 56 (01) :109-111

[5] Thin-layer chromatography mass spectrometry using activated carbon, surface-assisted laser desorption/ionization [J].

Chen, YC ;

Shiea, J ;

Sunner, J .

JOURNAL OF CHROMATOGRAPHY A, 1998, 826 (01) :77-86

[6] Thin layer chromatography/mass spectrometry [J].

Cheng, Sy-Chyi ;

Huang, Min-Zong ;

Shiea, Jentaie .

JOURNAL OF CHROMATOGRAPHY A, 2011, 1218 (19) :2700-2711

[7] Thin-Layer Chromatography/Laser-Induced Acoustic Desorption/Electrospray Ionization Mass Spectrometry [J].

Cheng, Sy-Chyi ;

Huang, Min-Zong ;

Shiea, Jentaie .

ANALYTICAL CHEMISTRY, 2009, 81 (22) :9274-9281

[8] MALDI-TOF-MS Directly Combined with TLC: A Review of the Current State [J].

Fuchs, Beate ;

Suess, Rosemarie ;

Nimptsch, Ariane ;

Schiller, Juergen .

CHROMATOGRAPHIA, 2009, 69 :S95-S105

[9] Analysis and quantitation of diazepam in cream biscuits by high-performance thin-layer chromatography and its confirmation by mass spectrometry [J].

Ghosh, P ;

Reddy, MMK ;

Ramteke, VB ;

Rao, BS .

ANALYTICA CHIMICA ACTA, 2004, 508 (01) :31-35

[10] Ambient Diode Laser Desorption Dielectric Barrier Discharge Ionization Mass Spectrometry of Nonvolatile Chemicals [J].

Gilbert-Lopez, Bienvenida ;

Schilling, Michael ;

Ahlmann, Norman ;

Michels, Antje ;

Hayen, Heiko ;

Molina-Diaz, Antonio ;

Garcia-Reyes, Juan F. ;

Franzke, Joachim .

ANALYTICAL CHEMISTRY, 2013, 85 (06) :3174-3182

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