Influence of ozonolysis time during sugarcane pretreatment: Effects on the fiber and enzymatic saccharification

被引:23
作者
Perrone, Olavo Micali [1 ,2 ]
Rossi, Jessika Souza [1 ]
de Souza Moretti, Marcia Maria [1 ]
Carreira Nunes, Christiane da Costa [1 ]
Bordignon, Sidnei Emilio [1 ]
Gomes, Eleni [1 ]
Da-Silva, Roberto [1 ]
Boscolo, Mauricio [1 ]
机构
[1] Univ Estadual Paulista, IBILCE, UNESP, Sao Paulo, Brazil
[2] Univ Estadual Paulista, IBILCE, UNESP, Dept Chem & Environm Sci, Sao Paulo, Brazil
关键词
Ozonolysis; Pretreatment; Sugarcane bagasse; Enzymatic hydrolysis; Cellulosic ethanol; BIOETHANOL PRODUCTION; ETHANOL-PRODUCTION; LIGNOCELLULOSIC BIOMASS; ALKALINE PRETREATMENT; ACID-HYDROLYSIS; BAGASSE; ULTRASOUND; RELEASE;
D O I
10.1016/j.biortech.2016.11.043
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
Modifications in sugarcane bagasse (SCB) from ozonolysis (O) NaOH (B) and ultrasound (U) (OBU) treatment for cellulosic ethanol production by enzymatic hydrolysis, were evaluated when increasing the exposure time of SCB to ozone. The lignin, cellulose, and hemicellulose after treatment were quantified: lignin removal and a consequent increase in cellulose content were shown using an infrared spectroscopic technique (ATR-FTIR) and chemical characterization. X-ray diffraction analysis (XRD) proved that OBU treatment does not affect the crystalline cellulose portion and electron microscopy techniques established that the fiber region most affected by the OBU treatment was the secondary cell wall, where the greatest lignin content is located. For OBU-60 treatment the lignin content was reduced and consequently there was a significant increase in cellulose content. After enzymatic hydrolysis, this pretreated SCB released 418 mg glucose/g, corresponding to six times more than untreated SCB and a yield of 93% of the cellulose available. (C) 2016 Elsevier Ltd. All rights reserved.
引用
收藏
页码:733 / 737
页数:5
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