Controlling the Uptake of Diarylethene-Based Cell-Penetrating Peptides into Cells Using Light

被引：7

作者：

Schober, Tim ^{[1
]}

Wehl, Ilona ^{[2
]}

Afonin, Sergii ^{[3
]}

Babii, Oleg ^{[3
]}

Iampolska, Anna ^{[4
,5
]}

Schepers, Ute ^{[2
]}

Komarov, Igor, V ^{[4
,6
]}

Ulrich, Anne S. ^{[1
,3
]}

机构：

[1] KIT, IOC, Fritz Haber Weg 6, D-76131 Karlsruhe, Germany

[2] KIT, Inst Funct Interfaces IFG, POB 3640, D-76021 Karlsruhe, Germany

[3] KIT, Inst Biol Interfaces IBG 2, POB 3640, D-76021 Karlsruhe, Germany

[4] Taras Shevchenko Natl Univ Kyiv, Vul Volodymyrska 60, UA-01601 Kiev, Ukraine

[5] Enamine Ltd, Vul Chervonotkatska 78, UA-02094 Kiev, Ukraine

[6] Lumobiotics GmbH, Auerstr 2, D-76227 Karlsruhe, Germany

来源：

CHEMPHOTOCHEM | 2019年 / 3卷 / 06期

基金：

欧盟地平线“2020”;

关键词：

cell-penetrating peptides; cell uptake; diarylethenes; photopharmacology; photoswitches; PROTEIN-PROTEIN INTERACTIONS; IN-VIVO; DRUG-DELIVERY; DESIGN; PHOTOCONTROL; CYCLOTIDES; DRIVEN;

D O I：

10.1002/cptc.201900019

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

We report on diarylethene (DAE)-containing photoswitchable cell-penetrating peptides (CPPs) capable of photocontrolled cell entry. We demonstrate in vitro that reversible photoisomerization of a DAE fragment in the backbones of non-cytotoxic cyclic peptides influences their binding to cell membranes and subsequent CPP-mediated cargo internalization. Benign red light can be used to activate cell entry of our compounds. We suggest that the observed differences in cell uptake of the two photoisomers are due to changes in the molecular flexibility achieved upon DAE photoisomerization.

引用

页码：384 / 391

页数：8

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