Application of antibody nanogold ionic liquid carbon paste electrode for sensitive electrochemical immunoassay of thyroid-stimulating hormone

被引：113
作者：
Beitollahi, Hadi [1 ]
Ivari, Susan Ghofrani [2 ]
Torkzadeh-Mahani, Masoud [3 ]
机构：
[1] Grad Univ Adv Technol, Environm Dept, Inst Sci & High Technol & Environm Sci, Kerman, Iran
[2] Grad Univ Adv Technol, Dept Chem, Kerman, Iran
[3] Grad Univ Adv Technol, Biotechnol Dept, Inst Sci & High Technol & Environm Sci, Kerman, Iran
来源：
BIOSENSORS & BIOELECTRONICS | 2018年 / 110卷
关键词：
Electrochemical immunoassay; Thyroid stimulating hormone; Carbon paste electrode; Gold nanoparticle; Ionic liquid; O-Aminophenol; CHEMILUMINESCENCE IMMUNOASSAY; AMPEROMETRIC IMMUNOSENSOR; GOLD NANOPARTICLES; GRAPHENE; ENZYME; NANOCOMPOSITE; PROSPECTS; SENSOR; POINT; CARE;
D O I：
10.1016/j.bios.2018.03.003
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
A novel electrochemical immunosensor based on carbon paste electrode (CPE) composed of ionic liquid (IL) and graphite was constructed. It demonstrated good efficiency for quick (each test in 30 s) determination of thyroid stimulating hormone (TSH). Electrode surface was modified by gold nanoparticles in order to immobilize of the thyroid stimulating hormone antibody (anti-TSH) on the CPE. The immunoassay structure was established by sandwiching the antigen (TSH) between the thyroid stimulating hormone antibody on the CPE surface modified with gold nanoparticles and the secondary antibody, polyclonal anti-human-TSH labeled with horseradish peroxidase (HRP-labeled anti-TSH). The signal of differential pulse voltammetry (DPV) was used as a basis for the determination of TSH concentration. This signal is generated by the reaction between O-aminophenol (OAP) and H2O2 catalyzed by HRP. The proposed immunosensor is able to measure the concentration of TSH in a linear range between 0.2 and 90.0 ng/mL with a detection limit 0.1 +/- 0.02 ng/mL. In addition, high sensitivity and acceptable stability were achieved by this immunosensor which is promising in the clinical assay of TSH.
引用
收藏
页码：97 / 102
页数：6
相关论文
共 39 条
[1]   Novel graphene modified carbon-paste electrode for promazine detection by square wave voltammetry [J].
AlAqad, Khaled M. ;
Suleiman, Rami ;
Al Hamouz, Othman Charles S. ;
Saleh, Tawfik A. .
JOURNAL OF MOLECULAR LIQUIDS, 2018, 252 :75-82
[2]   Electrochemical Sensor Based on Ionic Liquid Crystal Modified Carbon Paste Electrode in Presence of Surface Active Agents for Enoxacin Antibacterial Drug [J].
Atta, Nada F. ;
Galal, Ahmed ;
Azab, Shereen M. ;
Ibrahim, Asmaa H. .
JOURNAL OF THE ELECTROCHEMICAL SOCIETY, 2015, 162 (01) :B9-B15
[3]   A sensitive graphene and ethyl 2-(4-ferrocenyl-[1,2,3]triazol-1-yl) acetate modified carbon paste electrode for the concurrent determination of isoproterenol, acetaminophen, tryptophan and theophylline in human biological fluids [J].
Beitollahi, Hadi ;
Movlaee, Kaveh ;
Ganjali, Mohammad Reza ;
Norouzi, Parviz .
JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 2017, 799 :576-582
[4]   GO/Fe3O4@SiO2 core-shell nanocomposite-modified graphite screen-printed electrode for sensitive and selective electrochemical sensing of dopamine and uric acid [J].
Beitollahi, Hadi ;
Nejad, Fariba Garkani ;
Shakeri, Shahryar .
ANALYTICAL METHODS, 2017, 9 (37) :5541-5549
[5]   A magnetic core-shell Fe3O4@SiO2/MWCNT nanocomposite modified carbon paste electrode for amplified electrochemical sensing of amlodipine and hydrochlorothiazide [J].
Beitollahi, Hadi ;
Ebadinejad, Farshid ;
Shojaie, Fahimeh ;
Torkzadeh-Mahani, Masoud .
ANALYTICAL METHODS, 2016, 8 (32) :6185-6193
[6]   Voltammetric determination of 6-thioguanine and folic acid using a carbon paste electrode modified with ZnO-CuO nanoplates and modifier [J].
Beitollahi, Hadi ;
Ivari, Susan Ghofrani ;
Torkzadeh-Mahani, Masoud .
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, 2016, 69 :128-133
[7]   Enzymatically catalytic deposition of gold nanoparticles by glucose oxidase-functionalized gold nanoprobe for ultrasensitive electrochemical immunoassay [J].
Cheng, Hui ;
Lai, Guosong ;
Fu, Li ;
Zhang, Haili ;
Yu, Aimin .
BIOSENSORS & BIOELECTRONICS, 2015, 71 :353-358
[8]   Quantitative analysis of thyroid-stimulating hormone (TSH) using SERS-based lateral flow immunoassay [J].
Choi, Suji ;
Hwang, Joonki ;
Lee, Sangyeop ;
Lim, Dong Woo ;
Joo, Hoodon ;
Choo, Jaebum .
SENSORS AND ACTUATORS B-CHEMICAL, 2017, 240 :358-364
[9]   An electrochemical biosensor for α-fetoprotein based on carbon paste electrode constructed of room temperature ionic liquid and gold nanoparticles [J].
Ding, Caifeng ;
Zhao, Fei ;
Ren, Rui ;
Lin, Jin-Ming .
TALANTA, 2009, 78 (03) :1148-1154
[10]   Ultrasensitive Electrochemiluminescent Competitive Immunoassay for β-Adrenergic Agonist Salbutamol Based on Quantum Dots and Enzymatic Amplification [J].
Dong, Tiantian ;
Tang, Qinghui ;
Chen, Min ;
Deng, Anping ;
Li, Jianguo .
JOURNAL OF THE ELECTROCHEMICAL SOCIETY, 2016, 163 (03) :B62-B67
1234