Random amplified polymorphic DNA (RAPD) interpretation requires a sensitive method for the detection of amplified DNA

被引：16
|
作者：
Valentini, A [1 ]
Timperio, AM [1 ]
Cappuccio, I [1 ]
Zolla, L [1 ]
机构：
[1] UNIV TUSCIA,DIPARTIMENTO SCI AMBIENTALI,I-01100 VITERBO,ITALY
来源：
ELECTROPHORESIS | 1996年 / 17卷 / 10期
关键词：
random amplified polymorphic DNA; capillary electrophoresis; polymorphism; DNA;
D O I：
10.1002/elps.1150171009
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The random amplified polymorphic DNA technique (RAPD) has found wide use in molecular genetics because of its speed and ease of use. For various reasons, with this method the amplified DNA fragments are produced at different concentrations between genotypes and even between polymerase chain reaction (PCR) runs. Since the detection of the multiple amplified fragments is performed routinely by agarose gel, and seldom by acrylamide gel electrophoresis, we have found that by capillary zone electrophoresis (CZE), which is more sensitive and accurate than gel electrophoresis, it is possible to unequivocally detect amplified fragments even at low concentration, avoiding polymorphism misinterpretation. CZE is also useful to make more potentially polymorphic fragments evident per random primer used, with obvious economical benefits.
引用
收藏
页码：1553 / 1554
页数：2
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