Immobilized hydroxynitrile lyases for enantioselective synthesis of cyanohydrins: Sol-gels and cross-linked enzyme aggregates

被引：37
作者：
Cabirol, Fabien L.
Hanefeld, Ulf
Sheldon, Roger A.
机构：
[1] Delft Univ Technol, Lab Biocatalysis & Organ Chem, NL-2628 BL Delft, Netherlands
[2] Inst Chem & Engn Sci, Singapore 627833, Singapore
来源：
ADVANCED SYNTHESIS & CATALYSIS | 2006年 / 348卷 / 12-13期
关键词：
asymmetric synthesis; CLEA; cyanohydrins; hydroxynitrile lyase; oxynitrilase; sol-gel;
D O I：
10.1002/adsc.200606139
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
The hydroxynitrile lyases (HNLs) from Prunus amygdalus (PaHNL), Manihot esculenta (MeHNL), and Hevea brasiliensis (HbHNL) were successfully immobilized in sol-gels. The cross-linked enzyme aggregate (CLEA) of HbHNL was also prepared. These immobilized enzymes and the commercial PaHNL- and MeHNL-CLEAs were employed for the enantioselective synthesis of cyanohydrins. The sol-gels were highly efficient at low catalyst loading and particularly stable towards the organic solvent (diisopropyl ether) and substrate/product deactivation. The stabilization effect was inconsistent for CLEAs of different HNLs and significant deactivation of PaHNL- and HbHNL-CLEAs in diisopropyl ether was observed. In contrast commercial MeHNL-CLEA proved to be a remarkably robust and efficient biocatalyst in diisopropyl ether.
引用
收藏
页码：1645 / 1654
页数：10
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