HtrA1 regulates epithelial-mesenchymal transition in hepatocellular carcinoma

被引:14
作者
Zhu, Feng [1 ]
Duan, Yun-Fei [1 ]
Bao, Wan-Yuan [1 ]
Liu, Wen-Song [1 ]
Yang, Yue [1 ]
Cai, Hui-Hua [1 ]
机构
[1] Soochow Univ, Dept Hepatobiliary Surg, Affiliated Hosp 3, Changzhou 213003, Jiangsu, Peoples R China
关键词
HtrA1; EMT; Hepatocellular carcinoma; Metastasis; SERINE-PROTEASE HTRA1; CANCER METASTASIS; TUMOR; EXPRESSION; FAMILY; PROGNOSIS; ROLES; CELLS; SNAIL; EMT;
D O I
10.1016/j.bbrc.2015.09.105
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Background and aims: Epithelial mesenchymal transition (EMT) is involved in the development and progression of cancer. HtrA1 had been showed to play a modulatory role in metastasis of hepatocellular carcinoma (HCC). The relationship between HtrA1 and EMT in HCC was investigated in the present study. Methods: The HtrA1 expression in human HCC tumor tissues and cells was determined by real-time PCR. SiRNA-HtrA1 and pcDNA-HtrA1 were respectively transfected into HepG2 and MHCC97H cells to observe their effects on cell migration and expression of EMT-associated markers Vimentin and E-cadherin. The relationship between HtrA1 and EMT in 60 HCC patients was also investigated. Results: HtrA1 expression of tumor tissues was down-regulated with the increasing of number in lymph nodes metastasis in HCC patients. HtrA1 down-regulation led to the significant increase of cell migration, Vimentin expression and decrease of E-cadherin expression, while HtrA1 overexpression resulted in an opposite function. The HtrA1 expression was positively related to the E-cadherin level (R-2 = 0.5903, P < 0.001) and negatively correlated with Vimentin level (R-2 = 0.6067, P < 0.001) in tumor tissues of HCC, respectively. Conclusion: HtrA1 expression was closely related to EMT, which might be a potential mechanism underlying metastasis of HCC. (C) 2015 Elsevier Inc. All rights reserved.
引用
收藏
页码:589 / 594
页数:6
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