Metabolism of MDCK cells during cell growth and influenza virus production in large-scale microcarrier culture

被引：129

作者：

Genzel, Y

Behrendt, I

König, S

Sann, H

Reichl, U

机构：

[1] Max Planck Inst Dynam Complex Tech Syst, D-39106 Magdeburg, Germany

[2] Otto Von Guericke Univ, Lehrstuhl Bioprozesstech, D-39106 Magdeburg, Germany

来源：

VACCINE | 2004年 / 22卷 / 17-18期

关键词：

equine influenza virus; vaccine production; MDCK; microcarrier;

D O I：

10.1016/j.vaccine.2003.11.041

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The production of equine influenza in Madin-Darby canine kidney (MDCK) cells in large-scale microcarrier culture is described with detailed on- and off-line analytical data during cell growth and virus replication. Metabolite concentration profiles for glucose, glutamine, lactate and ammonium are shown. Lactate and armmonium concentrations were always below inhibiting levels. Concentration profiles for essential and non-essential amino acids of the cell culture medium are discussed. During cell growth proline was released into the medium with a significant rate while two amino acids, serine and methionine were almost depleted. After infection, virus titer increased after a delay of 10-16 h whereas first changes in amino acid metabolism could be observed within 4 h post-infection. Here, glutamate and aspartate increase correlated to virus release kinetics, indicating cell disruption and apoptosis. Starting with a moi of 0.025 resulted in a maximum virus yield of 2.4log HA/100 mul at 44 h post-infection. (C) 2003 Elsevier Ltd. All rights reserved.

引用

页码：2202 / 2208

页数：7

共 26 条

[1] Corneal organ culture: Effects of serum and a stabilised form of L-glutamine [J].