Amphiphilic inulin graft co-polymers as self-assembling micelles for doxorubicin delivery

This paper reports the synthesis and characterization of a new amphiphilic inulin graft copolymer able to self-assemble in water into a micelle type structure and to deliver the anticancer model drug doxorubicin. For this aim, inulin was chemically modified in the side chain with primary amine groups (INU-EDA) and these were used as reactive moieties for the conjugation of poly ethylene glycol 2000 and succinyl-ceramide. The CMC of obtained amphiphilic inulin derivatives (INU-ceramide and INU-ceramide-PEG(2000)) was measured by means of fluorescence analysis using pyrene as the fluorescent probe. The obtained micelles were characterized by DLS and AFM analysis and the ability to release the loaded doxorubicin was studied in different media. Finally the cytotoxicity profile on both cancer (HCT116) and normal (16 HBE) cell lines and in vitro ability to deliver the drug into cancer cells were evaluated.