A Method to Measure Trace Enzyme Based on Microchip Capillary Electrophoresis

被引：1

作者：

Cong Hui ^{[1
]}

Wang Hui-Min ^{[1
]}

Ju Shao-Qing ^{[1
]}

Jin Qing-Hui ^{[2
]}

Jia Chun-Ping ^{[2
]}

Song Hong-Wei ^{[1
]}

机构：

[1] Nantong Univ, Affiliated Hosp, Lab Med Ctr, Nantong 226001, Peoples R China

[2] Chinese Acad Sci, Shanghai Inst Microsyst & Informat Technol, Shanghai 200050, Peoples R China

来源：

PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS | 2009年 / 36卷 / 04期

关键词：

microchip capillary electrophoresis; nicotinamide adenine dinucleotide(NAD(+)); reduced nicotinamide adenine dinucleotide(NADH); lactate dehydrogenase; GEL-ELECTROPHORESIS; AMINO-ACIDS; SEPARATIONS; CHIP; DNA;

D O I：

10.3724/SP.J.1206.2008.00605

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The microchip fabricated by MEMS technology is successfully used to finish the electrophoresis process. A novel electrophoresis analysis protocol of the trace enzyme of lactate dehydrogenase (LDH) on microfluidic chip platform is developed with a Xe lamp-induced fluorescence detection system. Satisfactory separation of LDH was achieved in 75 mmol/L borate buffer containing 9.73 mu mol/L calcium lactate as running buffer (pH 9.4) within 4 min. The detection of LDH limits (S/N=3) is 6x10(-3) U/L. The coefficients of variation of peak time and areas were 5.32% and 3.17%. The method is easily operated. To application microfluidic chips with the method, the sensitive of detectable enzyme will be much more improved. The method for trace enzymes has potential for clinical application.

引用

页码：512 / 516

页数：5

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