Optimized shoot regeneration for Indian soybean: the influence of exogenous polyamines

A simple and efficient regeneration protocol was established for soybean [Glycine max (L.) Merrill]. Cotyledonary node explants from 7-day-old in vitro seedlings were used as explants. The effect of different plant growth regulators [N (6) -benzyladenine (BA), kinetin (KT), thidiazuron (TDZ), gibberellic acid (GA(3)), zeatin riboside (ZTR), indole-3-acetic acid (IAA), and indole-3-butyric acid (IBA)] along with polyamines (Spermidine, spermine, and putrescine) were investigated at different stages of regeneration using direct organogenesis system. Exogenous spermidine (137.69 mu M) in shoot induction medium containing optimal BA concentration (2.22 mu M) induced maximum number of shoots (39.02 shoots/explant) compared to BA (2.22 mu M) alone. Regenerated shoots elongated well in shoot elongation medium containing GA(3) (1.45 mu M) and spermine (74.13 mu M), and developed profuse roots in root induction medium containing putrescine (62.08 mu M). Rooted plantlets were successfully hardened and acclimatized with a survival rate of 92 %. The amenability of the standardized protocol using cultivar PK 416 was tested on four more Indian soybean cultivars JS 90-41, Hara soy, Co1, and Co2 of which PK 416 was found to be the best responding cultivar, with a maximum of 96.94 % shoot induction.