Volumetric structured illumination microscopy enabled by a tunable-focus lens

被引：18

作者：

Hinsdale, Taylor ^{[1
]}

Malik, Bilal H. ^{[1
]}

Olsovsky, Cory ^{[1
]}

Jo, Javier A. ^{[1
]}

Maitland, Kristen C. ^{[1
]}

机构：

[1] Texas A&M Univ, Dept Biomed Engn, College Stn, TX 77843 USA

来源：

OPTICS LETTERS | 2015年 / 40卷 / 21期

基金：

美国国家卫生研究院; 美国国家科学基金会;

关键词：

CONFOCAL LASER ENDOMICROSCOPY; IN-VIVO; LIGHT;

D O I：

10.1364/OL.40.004943

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We present a mechanical-scan-free method for volumetric imaging of biological tissue. The optical sectioning is provided by structured illumination, and the depth of the imaging plane is varied using an electrically tunable-focus lens. We characterize and evaluate the ability of this axial-scanning mechanism in structured illumination microscopy and demonstrate its ability to perform subcellular resolution imaging in oral mucosa ex vivo. The proposed mechanism can potentially convert any wide-field microscope to a 3D-imaging platform without the need for mechanical scanning of imaging optics and/or sample. (C) 2015 Optical Society of America

引用

页码：4943 / 4946

页数：4

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