Expression of intracellular calcium signalling genes in cattle skin during tick infestation

被引：22

作者：

Bagnall, N. ^{[1
,2
]}

Gough, J. ^{[1
]}

Cadogan, L. ^{[1
]}

Burns, B. ^{[2
,3
]}

Kongsuwan, K. ^{[1
,2
]}

机构：

[1] CSIRO Livestock Ind, St Lucia, Qld 4072, Australia

[2] Cooperat Res Ctr Beef Genet Technol, Armidale, NSW, Australia

[3] Cent QLD Mail Ctr, Trop Beef Ctr, Dept Primary Ind & Fisheries, Rockhampton, Qld, Australia

来源：

PARASITE IMMUNOLOGY | 2009年 / 31卷 / 04期

关键词：

calcium signalling; gene expression; R; microplus; tick resistance; T-CELL-ACTIVATION; BOOPHILUS-MICROPLUS; SALIVARY PROTEIN; HOST-RESISTANCE; CALRETICULIN; RESPONSES; DIFFERENTIATION; RECEPTOR; IMMUNOGLOBULIN; INHIBITION;

D O I：

10.1111/j.1365-3024.2008.01092.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

It is widely acknowledged that changes in intracellular calcium ion (Ca2+) concentration provide dynamic signals that control a plethora of cellular processes, including triggering and mediating host defence mechanisms. In this study, quantitative real-time PCR was used to analyse gene expression of 14 Ca2+ signalling proteins in skin obtained from high tick-resistant (HR) and low tick-resistant (LR) cattle following artificial challenge with cattle tick (Rhipicephalus (Boophilus) microplus). Up-regulation of numerous genes was observed in both HR and LR skin following tick challenge, however substantially higher transcription activation was found in HR tissue. The elevated expression in HR skin of specific Ca2+ signalling genes such as AHNAK, CASQ, IL2, NFAT2CIP and PLCG1 may be related to host resistance. Our data suggest that Ca2+ and its associated proteins might play an important role in host response to ticks and that further investigation is warranted.

引用

页码：177 / 187

页数：11

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