Suitability of IS6110-RFLP and MIRU-VNTR for Differentiating Spoligotyped Drug-Resistant Mycobacterium tuberculosis Clinical Isolates from Sichuan in China

被引：16

作者：

Zheng, Chao ^{[1
]}

Zhao, Yuding ^{[1
]}

Zhu, Guoqiang ^{[1
]}

Li, Song ^{[1
]}

Sun, Honghu ^{[1
]}

Feng, Qin ^{[1
,2
]}

Luo, Mei ^{[3
]}

Wu, Fanzi ^{[1
]}

Li, Xuefeng ^{[1
]}

Hill, Veronique ^{[4
]}

Rastogi, Nalin ^{[4
]}

Sun, Qun ^{[1
]}

机构：

[1] Sichuan Univ, Coll Life Sci, Minist Educ, Key Lab Bioresources & Ecoenvironm, Chengdu 610065, Sichuan, Peoples R China

[2] Chengdu Longquan Hlth Sch, Chengdu 610100, Sichuan, Peoples R China

[3] Chengdu AntiTB Hosp, Chengdu 610016, Sichuan, Peoples R China

[4] Inst Pasteur Guadeloupe, WHO Supranat TB Reference Lab, F-97183 Abymes, France

来源：

BIOMED RESEARCH INTERNATIONAL | 2014年 / 2014卷

关键词：

FRAGMENT-LENGTH-POLYMORPHISM; TANDEM-REPEAT LOCI; GENETIC DIVERSITY; BEIJING GENOTYPE; PULMONARY TUBERCULOSIS; STRAIN DIFFERENTIATION; MOLECULAR EPIDEMIOLOGY; DISCRIMINATORY ABILITY; SPREAD; INDIA;

D O I：

10.1155/2014/763204

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Genotypes of Mycobacterium tuberculosis complex (MTBC) vary with the geographic origin of the patients and can affect tuberculosis (TB) transmission. This study was aimed to further differentiate spoligotype-defined clusters of drug-resistant MTBC clinical isolates split in Beijing (n = 190) versus non-Beijing isolates (n = 84) fromSichuan region, the second high-burden province in China, by IS6110-restriction fragment length polymorphism (RFLP) and 24-locus MIRU-VNTRs. Among 274 spoligotyped isolates, the clustering ratio of Beijing family was 5.3% by 24-locus MIRU-VNTRs versus 2.1% by IS6110-RFLP, while none of the non-Beijing isolates were clustered by 24-locus MIRU-VNTRs versus 9.5% by IS6110-RFLP. Hence, neither the 24-locus MIRU-VNTR was sufficient enough to fully discriminate the Beijing family, nor the IS6110-RFLP for the non-Beijing isolates. A region adjusted scheme combining 12 highly discriminatory VNTR loci with IS6110-RFLP was a better alternative for typing Beijing strains in Sichuan than 24-locus MIRU-VNTRs alone. IS6110-RFLP was for the first time introduced to systematically genotype MTBC in Sichuan and we conclude that the region-adjusted scheme of 12 highly discriminative VNTRs might be a suitable alternative to 24-locus MIRU-VNTR scheme for non-Beijing strains, while the clusters of the Beijing isolates should be further subtyped using IS6110-RFLP for optimal discrimination.

引用

页数：11

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