Fatty acids potentiate interleukin-1β toxicity in the β-cell line INS-1E

被引：18
|
作者：
Aarnes, M [1 ]
Schonberg, S [1 ]
Grill, V [1 ]
机构：
[1] Norwegian Univ Sci & Technol, Dept Canc Res & Mol Biol, N-7034 Trondheim, Norway
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2002年 / 296卷 / 01期
关键词：
fatty acids; beta-cells; INS-1; interleukin-1; beta; cytokines; insulin biosynthesis;
D O I：
10.1016/S0006-291X(02)00819-7
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Evidence for "lipotoxicity," i.e., negative effects of fatty acids (FA) on pancreatic beta-cells is incomplete. Here, we tested whether non-toxic concentrations of FA potentiate toxic effects of interleukin-1beta (IL-1beta). Culture of INS-1E clonal beta-cells for 2-6 days with 70 muM docosahexaenoic acid (DHA), eicosapentaenoic acid, arachidonic acid, 0.1 mM linoleic acid, or 0.1-0.2 mM oleic acid exerted no or minor negative effects on cell viability (MTT assay). Viability was reduced by 0.5 ng/ml IL-1beta. All tested FA significantly aggravated this effect after 6 days of culture. 1L-1beta also exerted negative effects on cellular insulin content and DHA and oleic acid aggravated these effects. L-NAME, an inhibitor of constitutive nitric oxide (NO) synthase, reduced the negative effects of IL-1beta per se but did not abolish the potentiating effects of FA. Conclusion: FA potentiate toxic effects of IL-1beta on beta-cells by mechanisms that include NO-independent ones. (C) 2002 Elsevier Science (USA). All rights reserved.
引用
收藏
页码：189 / 193
页数：5
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