Overexpression of MLN51 triggers P-body disassembly and formation of a new type of RNA granules

被引：19

作者：

Cougot, Nicolas ^{[1
]}

Daguenet, Elisabeth ^{[2
]}

Baguet, Aurelie ^{[2
]}

Cavalier, Annie ^{[1
]}

Thomas, Daniel ^{[1
]}

Bellaud, Pascale

Fautrel, Alain ^{[3
]}

Godey, Florence ^{[3
,4
]}

Bertrand, Edouard ^{[5
]}

Tomasetto, Catherine ^{[2
]}

Gillet, Reynald ^{[1
]}

机构：

[1] Univ Rennes 1, IGDR, Translat & Folding Team, UMR CNRS 6290, F-35042 Rennes, France

[2] Univ Strasbourg, CNRS, INSERM, Inst Genet & Biol Mol & Cellulaire,UMR 7104,U964, F-67404 Illkirch Graffenstaden, France

[3] Univ Rennes 1, GFAS, Unite INSERM 991, Plateforme Histopathol,IFR 140, F-35043 Rennes, France

[4] Ctr Eugene Marquis, Ctr Ressources Biol Sante Rennes, F-35042 Rennes, France

[5] CNRS, Inst Mol Genet Montpellier, UMR 5535, F-34293 Montpellier 5, France

来源：

JOURNAL OF CELL SCIENCE | 2014年 / 127卷 / 21期

关键词：

Breast cancer; mRNA; MLN51; P-bodies; Stress granules; EXON-JUNCTION-COMPLEX; MESSENGER-RNA; BREAST-CANCER; PROCESSING BODIES; METASTATIC LYMPH-NODE-51; TRANSLATIONAL REPRESSION; MAMMALIAN-CELLS; STRESS GRANULES; CORE COMPLEX; DECAY;

D O I：

10.1242/jcs.154500

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Metastatic lymph node 51 (MLN51, also known as CASC3) is a core component of the exon junction complex (EJC), which is loaded onto spliced mRNAs and plays an essential role in determining their fate. Unlike the three other EJC core components [eIF4AIII, Magoh and Y14 (also known as RBM8A)], MLN51 is mainly located in the cytoplasm, where it plays a key role in the assembly of stress granules. In this study, we further investigated the cytoplasmic role of MLN51. We show that MLN51 is a new component of processing bodies (P-bodies). When overexpressed, MLN51 localizes in novel small cytoplasmic foci. These contain RNA, show directed movements and are distinct from stress granules and P-bodies. The appearance of these foci correlates with the process of P-body disassembly. A similar reduction in P-body count is also observed in human HER2-positive (HER2(+)) breast cancer cells overexpressing MLN51. This suggests that P-body disassembly and subsequent mRNA deregulation might correlate with cancer progression.

引用

页码：4692 / 4701

页数：10

共 43 条

[1] The Dynamics of Mammalian P Body Transport, Assembly, and Disassembly In Vivo [J].