Identification, expression and functional analysis of U3 snoRNA genes from Neurospora crassa

被引：1

作者：

Liu, Na ^{[1
]}

Huang, Qiaojuan ^{[1
]}

Hui, Zhou ^{[1
]}

Liang, Yintong ^{[1
]}

Yu, Chunhong ^{[1
]}

Qu, Lianghu ^{[1
]}

机构：

[1] Zhongshan Univ, State Key Lab Biocontrol, Minist Educ, Key Lab Gene Engn, Guangzhou 510275, Guangdong, Peoples R China

来源：

PROGRESS IN NATURAL SCIENCE-MATERIALS INTERNATIONAL | 2009年 / 19卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Neurospora crassa; U3; snoRNA; rRNA processing; SMALL NUCLEOLAR RNAS; YEAST SACCHAROMYCES-CEREVISIAE; PRE-RIBOSOMAL-RNA; FAMILIES; SEQUENCE; SITE; RIBONUCLEOPROTEINS; INTRONS; U8;

D O I：

10.1016/j.pnsc.2008.04.017

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

By screening of a cDNA library constructed with small RNAs from Neurospora crassa and genome analysis, three U3 snoRNAs 262, 270 and 275 nt were identified and named as NcU3A, NcU3B and NcU3C, respectively. Expression of the three U3 snoRNA was further analyzed by Northern blot hybridization. Meanwhile, two 5'-truncated U3 transcripts with different sizes were also identified, indicating a complex posttranscriptional processing of the U3 snoRNAs. In addition to the known functional elements to 18S rRNA, a novel antisense sequence to 26S rRNA was found in NcU3A. A primer extension assay showed that the sequence did not guide 2'-O-ribose methylation of 26S rRNA but probably functioned as an rRNA chaperon. In the N. crassa three U3 snoRNA genes are independently transcribed and each of them contains a small intron. The same intron-inserting site in the snoRNA genes among N. crassa and yeasts shows the ancient origin of the intron in these organisms. Our results provide new into the structural and functional diversity of U3 snoRNA family. (C) 2008 National Natural Science Foundation of China and Chinese Academy of Sciences. Published by Elsevier Limited and Science China Press. All rights reserved.

引用

页码：167 / 172

页数：6

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