Efficacy of timed embryo transfer with fresh and frozen in vitro produced embryos to increase pregnancy rates in heat-stressed dairy cattle

被引:96
作者
Ambrose, JD
Drost, M
Monson, RL
Rutledge, JJ
Leibfried-Rutledge, ML
Thatcher, MJ
Kassa, T
Binelli, M
Hansen, PJ
Chenoweth, PJ
Thatcher, WW [1 ]
机构
[1] Univ Florida, Dept Dairy & Poultry Sci, Gainesville, FL 32611 USA
[2] Univ Florida, Dept Large Anim Clin Sci, Gainesville, FL 32611 USA
[3] Univ Wisconsin, Dept Anim Sci, Madison, WI 53706 USA
[4] BOMED Inc, Madison, WI 53705 USA
关键词
heat stress; embryo transfer; timed insemination; pregnancy rate;
D O I
10.3168/jds.S0022-0302(99)75487-1
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
Our objective was to determine whether pregnancy rates in heat-stressed dairy cattle could be enhanced by timed embryo transfer of fresh (nonfrozen) or frozen-thawed in vitro-derived embryos compared to timed insemination. Ovulation in Holstein cows was synchronized by a GnRH injection followed 7 d later by PGF(2 alpha) and a second treatment with GnRH 48 h later. Control cows (n = 129) were inseminated 16 h (d 0) after the second GnRH injection. On d 7, a fresh (n = 133) or frozen-thawed (n = 142) in vitro-derived embryo was transferred to cows assigned for timed embryo transfer after categorizing the corpus luteum by palpation per rectum as 3 (excellent), 2 (good or fair), 1 (poor), and 0 (nonpalpable). Response to the synchronization treatment, determined by plasma progesterone concentration (ng/ml) less than or equal to 1.5 on d 0 and greater than or equal to 2.0 on d 7, was 76.2%. Mean plasma progesterone concentration on d 7 increased as the quality of corpus luteum improved from category 0 to 3. Concentrations of progesterone in plasma were elevated (greater than or equal to 2.0 ng/ml) at 21 d in 64.7 (fresh embryo), 40.3 (frozen embryo), and 41.4 +/- 0.1% (timed insemination) of cows, respectively. Cows that received a fresh embryo had a greater pregnancy rate at 45 to 52 d than did cows that received a frozen-thawed embryo or timed insemination (14.3 > 4.8, 4.9 +/- 2.3%). Body condition (d 0) of cows influenced the pregnancy rate and plasma progesterone concentrations. In summary, timed embryo transfer with fresh in vitro-produced embryos in heat-stressed dairy cattle improved pregnancy rate relative to timed insemination.
引用
收藏
页码:2369 / 2376
页数:8
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