Arbitrary multisite two-photon excitation in four dimensions

被引:39
作者
Daria, Vincent Ricardo [1 ,2 ]
Stricker, Christian [2 ]
Bowman, Richard [3 ]
Redman, Stephen [2 ]
Bachor, Hans-A. [1 ]
机构
[1] Australian Natl Univ, ARC Ctr Quantum Atom Opt, Canberra, ACT 0200, Australia
[2] Australian Natl Univ, John Curtin Sch Med Res, Canberra, ACT 0200, Australia
[3] Univ Glasgow, Dept Phys & Astron, SUPA, Glasgow G12 8QQ, Lanark, Scotland
基金
澳大利亚研究理事会;
关键词
LASER-SCANNING MICROSCOPY; FLUORESCENCE MICROSCOPY; MULTIPHOTON MICROSCOPY; OPTICAL TWEEZERS; HOLOGRAMS;
D O I
10.1063/1.3216581
中图分类号
O59 [应用物理学];
学科分类号
摘要
We demonstrate dynamic and arbitrary multisite two-photon excitation in three dimensions using the holographic projection method. Rapid response (fourth dimension) is achieved through high-speed noniterative calculation of the hologram using a video graphics accelerator board. We verify that the projected asymmetric spot configurations have sufficient spatiotemporal photon density for localized two-photon excitation. This system is a significant advance and can be applied to time-resolved photolysis of caged compounds in biological cells and complex neuronal networks, nonlinear microfabrication and volume holographic optical storage. (C) 2009 American Institute of Physics. [DOI: 10.1063/1.3216581]
引用
收藏
页数:3
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