Arbitrary multisite two-photon excitation in four dimensions

被引：39

作者：

Daria, Vincent Ricardo ^{[1
,2
]}

Stricker, Christian ^{[2
]}

Bowman, Richard ^{[3
]}

Redman, Stephen ^{[2
]}

Bachor, Hans-A. ^{[1
]}

机构：

[1] Australian Natl Univ, ARC Ctr Quantum Atom Opt, Canberra, ACT 0200, Australia

[2] Australian Natl Univ, John Curtin Sch Med Res, Canberra, ACT 0200, Australia

[3] Univ Glasgow, Dept Phys & Astron, SUPA, Glasgow G12 8QQ, Lanark, Scotland

来源：

APPLIED PHYSICS LETTERS | 2009年 / 95卷 / 09期

基金：

澳大利亚研究理事会;

关键词：

LASER-SCANNING MICROSCOPY; FLUORESCENCE MICROSCOPY; MULTIPHOTON MICROSCOPY; OPTICAL TWEEZERS; HOLOGRAMS;

D O I：

10.1063/1.3216581

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

We demonstrate dynamic and arbitrary multisite two-photon excitation in three dimensions using the holographic projection method. Rapid response (fourth dimension) is achieved through high-speed noniterative calculation of the hologram using a video graphics accelerator board. We verify that the projected asymmetric spot configurations have sufficient spatiotemporal photon density for localized two-photon excitation. This system is a significant advance and can be applied to time-resolved photolysis of caged compounds in biological cells and complex neuronal networks, nonlinear microfabrication and volume holographic optical storage. (C) 2009 American Institute of Physics. [DOI: 10.1063/1.3216581]

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