Heme oxygenase-1 induction protects murine cortical astrocytes from hemoglobin toxicity

被引：60

作者：

Regan, RF ^{[1
]}

Guo, YP ^{[1
]}

Kumar, N ^{[1
]}

机构：

[1] Thomas Jefferson Univ, Div Emergency Med, Philadelphia, PA 19107 USA

来源：

NEUROSCIENCE LETTERS | 2000年 / 282卷 / 1-2期

关键词：

iron; oxidation; free radical; central nervous system hemorrhage; stroke; cell culture;

D O I：

10.1016/S0304-3940(00)00817-X

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Exposure to micromolar concentrations of hemoglobin (Hb) results in the oxidative death of cultured cortical neurons, but glia are resistant. The role of heme oxygenase-1 (HO-1) induction on this glial resistance was investigated. Within two hours of exposure to 5 mu M Hb, immunoblotting demonstrated an increase in HO-1 in confluent glial cultures. Consistent with prior observations, 23-30 h Hb exposure had little or no effect on glial viability, as assessed by lactate dehydrogenase release. Concomitant treatment with the HO inhibitors tin protoporphyrin IX or the D-amino acid peptide rvnlrialry resulted in release of 40-71% of glial lactate dehydrogenase; protein synthesis inhibition with cycloheximide produced a similar effect. These results are consistent with the hypothesis that HO-1 induction protects cortical astrocytes from Hb toxicity. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：1 / 4

页数：4

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