Facile Immobilization of Enzyme via Co-Electrospinning: A Simple Method for Enhancing Enzyme Reusability and Monitoring an Activity-Based Organic Semiconductor

被引：21
作者：
Aldhahri, Musab M. [1 ,2 ]
Almulaiky, Yaaser Q. [4 ]
El-Shishtawy, Reda M. [3 ,5 ]
Al-Shawafi, Waleed [2 ]
Alngadh, Ahmed [6 ]
Maghrabi, Rayan [2 ]
机构：
[1] King Abdulaziz Univ, Ctr Nanotechnol, POB 80216, Jeddah 21589, Saudi Arabia
[2] King Abdulaziz Univ, Dept Biochem, POB 80200, Jeddah 21589, Saudi Arabia
[3] King Abdulaziz Univ, Dept Chem, POB 80200, Jeddah 21589, Saudi Arabia
[4] Univ Jeddah, Fac Sci, Dept Biochem, POB 80203, Jeddah 21589, Saudi Arabia
[5] Natl Res Ctr, Dept Dyeing Printing & Text Auxiliaries, Cairo 71516, Egypt
[6] King Abdulaziz City Sci & Technol, POB 6086, Riyadh 11442, Saudi Arabia
来源：
ACS OMEGA | 2018年 / 3卷 / 06期
关键词：
PHYTOSTEROL ESTERS; ALPHA-AMYLASE; LIPASE; MEMBRANES; NANOFIBERS; 8-HYDROXYQUINOLINE; STABILIZATION; NANOPARTICLES; STABILITY;
D O I：
10.1021/acsomega.8b00366
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The stability, reusability, and monitoring of enzyme activity have been investigated to improve their efficiency for successful utilization in a broad range of industrial and medical applications. Herein, we present a simple method for fabricating an electrospun fiber/enzyme scaffold via co-electrospinning. The characterization of soluble and immobilized alpha-amylases with regard to pH, thermal stability, and reusability were studied. An organic light emitting material tris(8-hydroxyquinoline) aluminum was incorporated to monitor the enzyme activity for several reuses.
引用
收藏
页码：6346 / 6350
页数：5
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