Production of acidic xylo-oligosaccharides by a family 10 endoxylanase from Thermoascus aurantiacus and use as plant growth regulators

被引：36
作者：
Katapodis, P
Kavarnou, A
Kintzios, S
Pistola, E
Kekos, D
Macris, BJ
Christakopoulos, P
机构：
[1] Natl Tech Univ Athens, Dept Chem Engn, Biotechnol Lab, Athens 15780, Greece
[2] Univ Agr, Fac Agr Biotechnol, Lab Plant Physiol, Athens 11855, Greece
来源：
BIOTECHNOLOGY LETTERS | 2002年 / 24卷 / 17期
关键词：
acidic oligosaccharides; plant growth regulators; xylanase;
D O I：
10.1023/A:1019898414801
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Neutral and acidic oligosaccharides were obtained from birchwood xylan by treatment with an endoxylanase, family 10 class, from Thermoascus aurantiacus. The main acidic xylooligosaccharide (aldotetrauronic acid) was separated from the hydrolysate by anion-exchange and size-exclusion chromatography and the primary structure was determined by C-13 NMR spectroscopy. The aldotetrauronic yield was 15% (w/w) of the total solubilised sugars. The addition of purified aldoterauronic acid at 1.6-16 mg l(-1) growth medium, induced callus and somatic embryogenesis in culture explants of common mallow (Malva silvestris L.) and cotton (Gosssypium hirsutum).
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页码：1413 / 1416
页数：4
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