Herpes simplex virus type 2 synergizes with interferon-gamma in the induction of nitric oxide production in mouse macrophages through autocrine secretion of tumour necrosis factor-alpha

被引:40
作者
Baskin, H [1 ]
EllermannEriksen, S [1 ]
Lovmand, J [1 ]
Mogensen, SC [1 ]
机构
[1] AARHUS UNIV,DEPT MED MICROBIOL & IMMUNOL,DK-8000 AARHUS C,DENMARK
关键词
D O I
10.1099/0022-1317-78-1-195
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
We have analysed the ability of herpes simplex virus type 2 (HSV-2) to induce nitric oxide (NO) production in resting BALB/c mouse peritoneal macrophages. In most experiments, macrophages produced very small amounts of NO upon infection with HSV-2. Mock virus preparations did not induce NO production, and virus inactivation experiments showed that infectious virus was required, Since interferon-gamma (IFN-gamma) is the prototype cytokine that is able to induce significant NO production in macrophages, we found it of interest to examine the influence of HSV-2 infection on the IFN-gamma-induced NO production, The virus exerted a synergistic effect on the IFN-gamma-induced NO release, which was accompanied by induction of the iNOS-gene as revealed by RT-PCR. This effect was largely dependent on the presence of infectious virus particles, since only a minor effect was seen with mock virus and inactivated virus preparations, From experiments with neutralizing antibodies to tumour necrosis factor-alpha (TNF-alpha) and IFN-alpha/beta it was concluded that the synergistic effect is dependent on autocrine secretion of TNF-alpha, which acts as a second signal and synergizes with IFN-gamma in NO production.
引用
收藏
页码:195 / 203
页数:9
相关论文
共 40 条
[31]   HERPES-SIMPLEX VIRUS TYPE-2 PRIMES MOUSE MACROPHAGES FOR AN EARLY AND GENETICALLY-DETERMINED RESPIRATORY BURST MEDIATED BY INTERFERON-ALPHA/BETA [J].
MOGENSEN, SC ;
ELLERMANNERIKSEN, S ;
SOMMERLUND, M .
JOURNAL OF GENERAL VIROLOGY, 1989, 70 :1371-1379
[32]  
MOGENSEN SC, 1988, RETICULOENDOTHELIAL, V10, P207
[33]  
MONCADA S, 1991, PHARMACOL REV, V43, P109
[34]   VIRUSES AND THE VERSATILE MACROPHAGE [J].
MORAHAN, PS ;
CONNOR, JR ;
LEARY, KR .
BRITISH MEDICAL BULLETIN, 1985, 41 (01) :15-21
[35]   NITRIC-OXIDE AS A SECRETORY PRODUCT OF MAMMALIAN-CELLS [J].
NATHAN, C .
FASEB JOURNAL, 1992, 6 (12) :3051-3064
[36]   GP120 HIV ENVELOPE GLYCOPROTEIN INCREASES THE PRODUCTION OF NITRIC-OXIDE IN HUMAN MONOCYTE-DERIVED MACROPHAGES [J].
PIETRAFORTE, D ;
TRITARELLI, E ;
TESTA, U ;
MINETTI, M .
JOURNAL OF LEUKOCYTE BIOLOGY, 1994, 55 (02) :175-182
[37]  
ROWLAND RR, 1994, J IMMUNOL, V132, P5785
[39]   INHIBITION OF HERPES-SIMPLEX VIRUS MULTIPLICATION BY ACTIVATED MACROPHAGES - A ROLE FOR ARGINASE [J].
WILDY, P ;
GELL, PGH ;
RHODES, J ;
NEWTON, A .
INFECTION AND IMMUNITY, 1982, 37 (01) :40-45
[40]  
WU L, 1992, CURR TOP MICROBIOL, V179, P89