Role of Nitric Oxide in the Change of 5-Hydroxytryptamine Synthesis in the Intestine by a Consecutive Administration of Methotrexate to Rats

被引：5

作者：

Machida, Takuji ^{[1
]}

Inotani, Akari ^{[1
]}

Shiga, Saki ^{[1
]}

Kon, Shuto ^{[1
]}

Yanada, Takumi ^{[1
]}

Kobayashi, Hiroya ^{[1
]}

Hamaue, Naoya ^{[2
]}

Hirafuji, Masahiko ^{[1
]}

Iizuka, Kenji ^{[1
]}

机构：

[1] Hlth Sci Univ Hokkaido, Sch Pharmaceut Sci, Dept Pharmacol Sci, 1757 Kanazawa, Ishikari, Hokkaido 0610293, Japan

[2] Hlth Sci Univ Hokkaido, Sch Pharmaceut Sci, Dept Mol Biosci, Ishikari, Hokkaido, Japan

来源：

PHARMACOLOGY | 2020年 / 105卷 / 11-12期

关键词：

Methotrexate; 5-Hydroxytryptamine; Tryptophan hydroxylase; Substance P; Small intestine; ENTEROCHROMAFFIN CELLS; METABOLISM; TOXICITY; RELEASE; MUCOSA;

D O I：

10.1159/000508973

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

This study aimed to investigate whether the consecutive administration of methotrexate affects 5-hydroxytryptamine (5-HT) synthesis in the rat small intestine. Rats received methotrexate at a dose of 12.5 mg/kg intraperitoneally on 4 consecutive days. N-G-nitro-L-arginine methyl ester (L-NAME) was given subcutaneously to inhibit nitric oxide (NO) synthase. Methotrexate moderately altered 5-HT synthesis, whereas the combined administration of methotrexate and L-NAME significantly changed 5-HT synthesis in the rat ileal tissue. These results suggest that endogenous NO has an antagonistic role in the induction of 5-HT synthesis in rats following the consecutive administration of methotrexate.

引用

页码：723 / 728

页数：6

共 22 条

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