Stable carbon isotope analysis of different tissues of beef animals in relation to their diet

被引:49
作者
De Smet, S
Balcaen, A
Claeys, E
Boeckx, P
Van Cleemput, O
机构
[1] State Univ Ghent, Fac Agr & Appl Biol Sci, Dept Anim Prod, B-9090 Melle, Belgium
[2] State Univ Ghent, ISOFYS, Lab Appl Phys Chem, B-9000 Ghent, Belgium
关键词
D O I
10.1002/rcm.1471
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
As part of a larger experiment, 31 young bulls, divided into three groups, were given different diets containing either C-3 plants or a combination of C-3 and C-4 plant-based feeds in three feeding periods before slaughter. Variation in the proportion of C-4 plant material in the diets was made by including or not maize or maize-derived ingredients, whereas the other dietary constituents were from C-3 plants. Analysis of stable carbon isotope ratios (delta(13)C value) was performed on different tissues taken at slaughter: blood, plasma, liver, kidney fat, hair, muscle and ruminal contents. Blood and plasma samples were also taken at the beginning of each period. A highly significant difference was found in the delta(13)C values of blood and plasma samples taken from animals that had received a diet of only C-3 plants or with 59% C-4 material for 70 days. The delta(13)C values of all different samples taken at slaughter were highly significantly different between the three feeding groups that had received diets with 0, 13.5 or 35% C-4 material for on average 137, 139 and 83 days, respectively. For the three groups, samples of hair, muscle, plasma, whole blood and liver were significantly enriched in C-13 compared with the diet (except for liver in one group), whereas kidney fat was significantly depleted. The proportion of C-4 plant material could be accurately estimated from the delta(13)C values of different tissue samples. Stable carbon analysis of different tissues from beef animals can be used to trace back diets containing variable proportions of C-3 and C-4 plant material. Copyright (C) 2004 John Wiley Sons, Ltd.
引用
收藏
页码:1227 / 1232
页数:6
相关论文
共 17 条
[1]   INFLUENCE OF DIET ON DISTRIBUTION OF CARBON ISOTOPES IN ANIMALS [J].
DENIRO, MJ ;
EPSTEIN, S .
GEOCHIMICA ET COSMOCHIMICA ACTA, 1978, 42 (05) :495-506
[2]   MECHANISM OF CARBON ISOTOPE FRACTIONATION ASSOCIATED WITH LIPID-SYNTHESIS [J].
DENIRO, MJ ;
EPSTEIN, S .
SCIENCE, 1977, 197 (4300) :261-263
[3]   Use of isotope analysis to characterize meat from Iberian-breed swine [J].
González-Martin, I ;
González-Pérez, C ;
Méndez, JH ;
Marqués-Macias, E ;
Poveda, FS .
MEAT SCIENCE, 1999, 52 (04) :437-441
[4]   Use of stable isotopes to determine diets of living and extinct bears [J].
Hilderbrand, GV ;
Farley, SD ;
Robbins, CT ;
Hanley, TA ;
Titus, K ;
Servheen, C .
CANADIAN JOURNAL OF ZOOLOGY-REVUE CANADIENNE DE ZOOLOGIE, 1996, 74 (11) :2080-2088
[5]   Tracing origins and migration of wildlife using stable isotopes: a review [J].
Hobson, KA .
OECOLOGIA, 1999, 120 (03) :314-326
[6]   ESTIMATION OF THE PROPORTION OF C-3 AND C-4 PLANT SPECIES IN THE DIET OF ANIMALS FROM THE RATIO OF NATURAL C-12 AND C-13 ISOTOPES IN THE FECES [J].
JONES, RJ ;
LUDLOW, MM ;
TROUGHTON, JH ;
BLUNT, CG .
JOURNAL OF AGRICULTURAL SCIENCE, 1979, 92 (FEB) :91-100
[7]  
JONES RJ, 1981, SEARCH, V12, P85
[8]   Measurement of stable isotope abundances in milk and milk ingredients - A possible tool for origin assignment and quality control [J].
Kornexl, BE ;
Werner, T ;
Rossmann, A ;
Schmidt, HL .
ZEITSCHRIFT FUR LEBENSMITTEL-UNTERSUCHUNG UND-FORSCHUNG A-FOOD RESEARCH AND TECHNOLOGY, 1997, 205 (01) :19-24
[9]   DEPENDENCE OF THE CARBON-ISOTOPE CONTENTS OF BREATH CARBON-DIOXIDE, MILK, SERUM AND RUMEN FERMENTATION PRODUCTS ON THE DELTA-C-13 VALUE OF FOOD IN DAIRY-COWS [J].
METGES, C ;
KEMPE, K ;
SCHMIDT, HL .
BRITISH JOURNAL OF NUTRITION, 1990, 63 (02) :187-196
[10]   CARBON ISOTOPE FRACTIONATION IN PLANTS [J].
OLEARY, MH .
PHYTOCHEMISTRY, 1981, 20 (04) :553-567