Evaluation of anti-inflammatory, antimicrobial and wound healing activity of Morus nigra

The present study was aimed to judge wound healing and the anti-inflammatory potential of Morus nigra leaves (MNL) in rats by incision and excision wound methods. Anti-inflammatory effects were assessed by measurement of paw oedema in carrageenan-induced inflammation in rats. The tensile strength and biochemical parameters were used for studying the wound healing properties of MNL by incision wound methodology. The wound contraction area, antioxidant status, and antimicrobial studies were exhausted excision wound methodology. Tensile strength and hydroxyproline level of different concentrations (% w/w) of MNL ointment treated groups were found significantly (P<.01) comparable to the reference group. There was a significant decreased in percent inhibition of paw oedema with an increase in MNL concentration. Moreover, MNL showed significant antimicrobial and antioxidant properties, by its ability to increase antioxidant levels (29.97 +/- 1.31 mg/50 mg tissue of SOD, 25.17 +/- 0.91 mmol/50 mg tissue of CAT and 31.71 +/- 1.21 mmol/50 mg tissue of GSH) and antimicrobial levels (16.0 +/- 0.50 mu g/disc for Bacillus subtilis; 16.1 +/- 0.28 mu g/disc for Staphylococcus aureus; 25.8 +/- 0.50 mu g/disc for Escherichia coli; 26.1 +/- 0.28 mu g/disc for Proteus vulgaris; 18.0 +/- 0.50 mu g/disc for Candida albicans and 17.3 +/- 0.50 mu g/disc for Candida glaborata). The results obtained in the present study clarify that MNL inhibits paw oedema and accelerates cutaneous wound healing. (C) 2019 SAAB. Published by Elsevier B.V. All rights reserved.