A novel detection method of human serum albumin based on CuInZnS quantum dots-Co2+ sensing system

被引：23

作者：

Gui, Wenying ^{[1
]}

Chen, Xueqian ^{[1
]}

Ma, Qiang ^{[1
]}

机构：

[1] Jilin Univ, Dept Analyt Chem, Coll Chem, Qianwei Rd 10, Changchun 130012, Jilin, Peoples R China

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2017年 / 409卷 / 15期

基金：

中国国家自然科学基金;

关键词：

CuInZnS QDs; Human serumalbumin (HSA); Turn off-on; Fluorescence assay; AQUEOUS SYNTHESIS; DOTS; ZNIN2S4;

D O I：

10.1007/s00216-017-0332-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We developed a novel "turn off-on" sensor for human serum albumin (HSA) detection based on CuInZnS quantum dots (CIZS QDs). The photoluminescence (PL) of QDs can be "turned off" by Co(II) first. Because of the strong binding ability of HSA with Co2+, Co2+ can be removed from CIZS QDs with the addition of HSA. As a result, the PL of CIZS QDs probe can be "turned on" with an increased concentration of HSA over a wide range. The analyte HSA concentration had a proportional linear relationship with the recovered PL intensity of CIZS QDs. The detection limit for HSA was 4.5 x 10(-8) mol L-1. The results indicated that the CIZS QDs- Co2+-BSA sensing system possessed higher sensitivity and better practicability for HSA detection.

引用

页码：3871 / 3876

页数：6

共 25 条

[1]

Carolina B, 2015, J PHARMACEUT BIOMED, V10, P186

[2] Low-Temperature and Template-Free Synthesis of ZnIn2S4 Microspheres [J].

Chen, Zhixin ;

Li, Danzhen ;

Zhang, Wenjuan ;

Chen, Chun ;

Li, Wenjuan ;

Sun, Meng ;

He, Yunhui ;

Fu, Xianzhi .

INORGANIC CHEMISTRY, 2008, 47 (21) :9766-9772

[3] Development and validation of a high-performance liquid chromatography (HPLC) method for the determination of human serum albumin (HSA) in medical devices [J].

Eertmans, Frank ;

Bogaert, Veerle ;

Puype, Barbara .

ANALYTICAL METHODS, 2011, 3 (06) :1296-1302

[4]

Escosura-Muniz A, 2006, ANAL BIOANAL CHEM, V2, P742

[5] Shape-controlled synthesis of ternary chalcogenide ZnIn2S4 and CuIn(S,Se)2 nano-/microstructures via facile solution route [J].

Gou, Xinglong ;

Cheng, Fangyi ;

Shi, Yunhui ;

Zhang, Li ;

Peng, Shengjie ;

Chen, Jun ;

Shen, Panwen .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (22) :7222-7229

[6] An overview of recent advances in quantum dots for biomedical applications [J].

He, Xuewen ;

Ma, Nan .

COLLOIDS AND SURFACES B-BIOINTERFACES, 2014, 124 :118-131

[7] Potentials and pitfalls of fluorescent quantum dots for biological imaging [J].

Jaiswal, JK ;

Simon, SM .

TRENDS IN CELL BIOLOGY, 2004, 14 (09) :497-504

[8] Cluster-Seeded Synthesis of Doped CdSe:Cu4 Quantum Dots [J].

Jawaid, Ali M. ;

Chattopadhyay, Soma ;

Wink, Donald J. ;

Page, Leah E. ;

Snee, Preston T. .

ACS NANO, 2013, 7 (04) :3190-3197

[9]

Jiang C, 2004, ANAL LETT, V6, P1129

[10] Spectrofluorimetric determination of human serum albumin using a doxycycline-europium probe [J].

Jiang, CQ ;

Luo, L .

ANALYTICA CHIMICA ACTA, 2004, 506 (02) :171-175

← 1 2 3 →