Electrocatalytic detection of thiols using an edge plane pyrolytic graphite electrode

被引：150

作者：

Moore, RR ^{[1
]}

Banks, CE ^{[1
]}

Compton, RG ^{[1
]}

机构：

[1] Univ Oxford, Phys & Theoret Chem Lab, Oxford OX1 3QZ, England

来源：

ANALYST | 2004年 / 129卷 / 08期

关键词：

D O I：

10.1039/b406276k

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The first example of using an edge plane pyrolytic graphite electrode in electroanalysis is reported as the determination of homocysteine, N-acetylcysteine, cysteine and glutathione is studied. The response of the electrode in the direct oxidation of thiol moieties is explored and found to be electrocatalytic producing a reduction in the overpotential while having enhanced signal-to-noise characteristics compared to glassy carbon and basal plane pyrolytic graphite electrodes. The effectiveness of the methodology is examined in the determination of cysteine species in a growth tissue media that contains a high number of common biological interferences. The advantageous properties of this electrode for thiol determination lie in its excellent catalytic activity, sensitivity and simplicity.

引用

页码：755 / 758

页数：4

共 28 条

[1] METAL-IONS AND COMPLEXES AS MODULATORS OF PROTEIN INTERFACIAL ELECTRON-TRANSPORT AT GRAPHITE-ELECTRODES [J].

ARMSTRONG, FA ;

COX, PA ;

HILL, HAO ;

LOWE, VJ ;

OLIVER, BN .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1987, 217 (02) :331-366

[2] APPLICATION OF CATALYTIC STRIPPING VOLTAMMETRY FOR THE DETERMINATION OF ORGANIC SULFUR-COMPOUNDS AT A HANGING MERCURY DROP ELECTRODE - BEHAVIOR OF CYSTEINE, CYSTINE AND N-ACETYLCYSTEINE IN THE PRESENCE OF NICKEL ION [J].

BANICA, FG ;

MOREIRA, JC ;

FOGG, AG .

ANALYST, 1994, 119 (02) :309-318

[3] CATALYTIC CATHODIC STRIPPING VOLTAMMETRY AT A HANGING MERCURY DROP ELECTRODE OF GLUTATHIONE IN THE PRESENCE OF NICKEL ION [J].

BANICA, FG ;

FOGG, AG ;

MOREIRA, JC .

ANALYST, 1994, 119 (11) :2343-2349

[4]

BANKS CE, 2004, IN PRESS CHEM COMMUN

[5] The electrooxidation of sulfur-containing compounds at boron-doped diamond electrode [J].

Chailapakul, O ;

Aksharanandana, P ;

Frelink, T ;

Einaga, Y ;

Fujishima, A .

SENSORS AND ACTUATORS B-CHEMICAL, 2001, 80 (03) :193-201

[6] Electrochemical detection of carbohydrates at carbon-nanotube modified glassy-carbon electrodes [J].

Deo, RP ;

Wang, J .

ELECTROCHEMISTRY COMMUNICATIONS, 2004, 6 (03) :284-287

[7] Fast-scan cyclic voltammetry of protein films on pyrolytic graphite edge electrodes: Characteristics of electron exchange [J].

Hirst, J ;

Armstrong, FA .

ANALYTICAL CHEMISTRY, 1998, 70 (23) :5062-5071

[8] LIQUID-CHROMATOGRAPHY WITH ELECTROCATALYTIC DETECTION OF CYSTEINE, N-ACETYLCYSTEINE AND GLUTATHIONE BY A PRUSSIAN BLUE FILM-MODIFIED ELECTRODE [J].

HOU, WY ;

WANG, EK .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1991, 316 (1-2) :155-163

[9]

Jacobsen DW, 1998, CLIN CHEM, V44, P1833

[10] Status of glutathione and other thiols and disulfides in human plasma [J].

Kleinman, WA ;

Richie, JP .

BIOCHEMICAL PHARMACOLOGY, 2000, 60 (01) :19-29

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