Morphological and mechanical properties of caudal vertebrae in the SAMP6 mouse model of senile osteoporosis

被引:37
作者
Silva, MJ [1 ]
Brodt, MD [1 ]
Uthgenannt, BA [1 ]
机构
[1] Washington Univ, Barnes Jewish Hosp, Dept Orthopaed Surg, Orthopaed Res Labs, St Louis, MO 63110 USA
关键词
senile osteoporosis; mouse model; bone strength; bone density; compression testing; vertebra;
D O I
10.1016/j.bone.2004.03.027
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The senescence-accelerated mouse, strain P6 (SAMP6), is a model of senile osteoporosis with relatively low bone mineral density (BMD), low rates of bone formation and reduced long-bone bending strength. Seeking to extend previous descriptions of the SAMP6 skeletal phenotype, we assessed the morphological and mechanical properties of vertebrae from SAM mice at 4 and 12 months of age. We hypothesized that, relative to SAMR1 controls, vertebrae from SAMP6 mice have: (1) less trabecular bone, (2) increased endosteal and periosteal bone size and (3) decreased whole-bone strength. Caudal vertebrae from adult female mice (SAMR1 and SAMP6; 4 and 12 months; n = 10-11 per group) were evaluated by micro-computed tomography and mechanical compression testing. SAMP6 vertebrae had 33% less trabecular bone volume in cephalad and caudal end regions than SAMR1 (P < 0.001) due to significant reductions in both trabecular number (P = 0.002) and thickness (P < 0.001). In contrast to previous findings in SAMP6 long bones, SAMP6 vertebrae showed no evidence of increased overall bone dimensions compared to SAMR1, and in fact had smaller total bone area (TA) and endosteal area (EA) at 12 months of age. Whereas SAMR1 vertebrae showed signs of age-related expansion from 4 to 12 months, SAMP6 vertebral dimensions did not change with age. Mechanical properties of SAMP6 vertebrae were not significantly different than SAMR1 vertebrae (stiffness, yield force, ultimate force, displacement at ultimate force, energy to ultimate force; P > 0.05), another finding in contrast to previous results in SAMP6 long bones. In summary, reduced vertebral trabecular bone volume is another feature of the SAMP6 mice with relevance to senile osteoporosis. However, age-related bone expansion and reduced whole-bone strength were not evident in SAMP6 vertebrae, indicating that while the SAMP6 mouse has many features relevant to senile osteoporosis in humans, not ail features are observed or detectable at all skeletal sites. (C) 2004 Elsevier Inc. All rights reserved.
引用
收藏
页码:425 / 431
页数:7
相关论文
共 18 条
  • [1] Genetic variability in adult bone density among inbred strains of mice
    Beamer, WG
    Donahue, LR
    Rosen, CJ
    Baylink, DJ
    [J]. BONE, 1996, 18 (05) : 397 - 403
  • [2] BELL G. H., 1967, CALCIFIED TISSUE RES, V1, P75, DOI 10.1007/BF02008077
  • [3] AGE-RELATED DIFFERENCES IN CROSS-SECTIONAL GEOMETRY OF THE FOREARM BONES IN HEALTHY WOMEN
    BOUXSEIN, ML
    MYBURGH, KH
    VANDERMEULEN, MCH
    LINDENBERGER, E
    MARCUS, LR
    [J]. CALCIFIED TISSUE INTERNATIONAL, 1994, 54 (02) : 113 - 118
  • [4] Growing C57B1/6 mice increase whole bone mechanical properties by increasing geometric and material properties
    Brodt, MD
    Ellis, CB
    Silva, MJ
    [J]. JOURNAL OF BONE AND MINERAL RESEARCH, 1999, 14 (12) : 2159 - 2166
  • [5] Age- and gender-related differences in vertebral bone mass, density, and strength
    Ebbesen, EN
    Thomsen, JS
    Beck-Nielsen, H
    Nepper-Rasmussen, HJ
    Mosekilde, L
    [J]. JOURNAL OF BONE AND MINERAL RESEARCH, 1999, 14 (08) : 1394 - 1403
  • [6] Linkage of decreased bone mass with impaired osteoblastogenesis in a murine model of accelerated senescence
    Jilka, RL
    Weinstein, RS
    Takahashi, K
    Parfitt, AM
    Manolagas, SC
    [J]. JOURNAL OF CLINICAL INVESTIGATION, 1996, 97 (07) : 1732 - 1740
  • [7] Phenotypic characterization of mice bred for high and low peak bone mass
    Klein, RF
    Shea, M
    Gunness, ME
    Pelz, GB
    Belknap, JK
    Orwoll, ES
    [J]. JOURNAL OF BONE AND MINERAL RESEARCH, 2001, 16 (01) : 63 - 71
  • [8] Reduced expression of interleukin-11 in bone marrow stromal cells of senescence-accelerated mice (SAMP6): Relationship to osteopenia with enhanced adipogenesis
    Kodama, Y
    Takeuchi, Y
    Suzawa, M
    Fukumoto, S
    Murayama, H
    Yamato, H
    Fujita, T
    Kurokawa, T
    Matsumoto, T
    [J]. JOURNAL OF BONE AND MINERAL RESEARCH, 1998, 13 (09) : 1370 - 1377
  • [9] AGE AND SEX-RELATED CHANGES IN STRUCTURE AND STRENGTH OF HUMAN FEMORAL-SHAFT
    MARTIN, RB
    ATKINSON, PJ
    [J]. JOURNAL OF BIOMECHANICS, 1977, 10 (04) : 223 - 231
  • [10] NORMAL VERTEBRAL BODY SIZE AND COMPRESSIVE STRENGTH - RELATIONS TO AGE AND TO VERTEBRAL AND ILIAC TRABECULAR BONE COMPRESSIVE STRENGTH
    MOSEKILDE, L
    MOSEKILDE, L
    [J]. BONE, 1986, 7 (03) : 207 - 212