Synthetic peptides for the immunodiagnosis of hepatitis A virus infection

被引：5

作者：

Gauna, A. ^{[1
]}

Losada, S. ^{[1
]}

Lorenzo, M. ^{[1
]}

Bermudez, H. ^{[1
]}

Toledo, M. ^{[2
]}

Perez, H. ^{[3
]}

Chacon, E. ^{[3
]}

Noya, O. ^{[1
,4
]}

机构：

[1] UCV, Fac Med, Inst Trop Med, Secc Biohelmintiasis, Caracas, Venezuela

[2] Cent Univ Venezuela, Escuela Med Luis Razetti, Catedra Parasitol, Caracas, Venezuela

[3] Inst Nacl Higiene INH Rafael Rangel, Dept Virol, Caracas, Venezuela

[4] Minist Poder Popular Salud, Inst Altos Estudios Dr Arnoldo Gabaldon INH, Ctr Estudios Malaria, Caracas, Venezuela

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 2015年 / 427卷

关键词：

Virus; Hepatitis A; Spot synthesis; Peptides; MABA; Immunodiagnosis; VIRAL-HEPATITIS; SPOT SYNTHESIS; PROTEIN; VP1; EPIDEMIOLOGY; ANTIGENS;

D O I：

10.1016/j.jim.2015.08.013

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

VP1, VP2 and VP3 molecules of hepatitis A virus are exposed capsid proteins that have shown to be antigenic and are used for diagnosis in recombinant-antigen commercial kits. In this study, we developed a sequence analysis in order to predict diagnostic peptide epitopes, followed by their spot synthesis on functionalized cellulose paper (Pepscan). This paper with synthetic peptides was tested against a sera pool of hepatitis A patients. Two peptide sequences, that have shown an antigenic recognition, were selected for greater scale synthesis on resin. A dimeric form of one of these peptides (IMT-1996), located in the C-Terminus region of protein VP1, was antigenic with a recognition frequency of 87-100% of anti-IgG antibodies and 100% of anti-IgM antibodies employing the immunological assays MABA and ELISA. We propose peptide IMT-1996, with less than twenty residues, as a cheaper alternative for prevalence studies and diagnosis of hepatitis A infection. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：1 / 5

页数：5

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