Intragraft FOXP3 Protein or mRNA During Acute Renal Allograft Rejection Correlates With Inflammation, Fibrosis, and Poor Renal Outcome

被引：23
作者：
Yapici, Unsal [1 ]
Bemelman, Frederike J. [2 ]
Scheepstra, Cornelis G. [1 ]
Roelofs, Joris J. T. H. [1 ]
Claessen, Nike [1 ]
van der Loos, Chris [1 ]
van Donselaar-van der Pant, Karlijn [2 ]
Bouts, Antonia H. M. [3 ]
Idu, Mirza M. [4 ]
Rowshani, Ajda T. [2 ]
ten Berge, Ineke J. M. [2 ]
Florquin, Sandrine [1 ]
机构：
[1] Univ Amsterdam, Acad Med Ctr, Dept Pathol, NL-1100 DD Amsterdam, Netherlands
[2] Univ Amsterdam, Acad Med Ctr, Dept Nephrol, NL-1100 DD Amsterdam, Netherlands
[3] Univ Amsterdam, Acad Med Ctr, Emma Childrens Hosp, NL-1100 DD Amsterdam, Netherlands
[4] Univ Amsterdam, Acad Med Ctr, Dept Vasc Surg, NL-1100 DD Amsterdam, Netherlands
来源：
TRANSPLANTATION | 2009年 / 87卷 / 09期
关键词：
Renal transplantation; Acute rejection; Regulatory T cells; TGF-beta; Inflammation; Fibrosis; T-CELLS; TGF-BETA; EXPRESSION; TRANSPLANT;
D O I：
10.1097/TP.0b013e3181a24a4b
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Background: Forkhead box (FOXP3) is considered to be a specific marker for regulatory T cells. The aim of this study was to correlate intragraft FOXP3 at mRNA and cellular levels during renal allograft rejection to response to therapy and late clinical outcome. Methods: Immunostainings and quantitative reverse-transcriptase polymerase chain reaction for FOXP3, CD3, and transforming growth factor (TGF)-beta were performed and results were related to histopathologic and clinical outcome. Results: A good correlation between immunohistochemical analysis and mRNA levels for both CD3 and FOXP3 was observed. Intragraft FOYP3 was significantly related to tubulitis and interstitial fibrosis. A strong correlation was found between FOXP3 and CD3 mRNA and between FOXP3 and TGF-beta mRNA. No correlation was found between FOXP3 and response to therapy. Discussion: In conclusion, intragraft FOXP3 at both cellular and molecular levels parallels T-cell infiltration during acute rejection. FOXP3 does not predict response to antirejection therapy. FOXP3 correlates with renal fibrosis, TGF-beta, and poor late renal outcome.
引用
收藏
页码：1377 / 1380
页数：4
相关论文
共 25 条
  • [1] Activation-induced FOXP3 in human T effector cells does not suppress proliferation or cytokine production
    Allan, Sarah E.
    Crome, Sarah Q.
    Crellin, Natasha K.
    Passerini, Laura
    Steiner, Theodore S.
    Bacchetta, Rosa
    Roncarolo, Maria G.
    Levings, Megan K.
    [J]. INTERNATIONAL IMMUNOLOGY, 2007, 19 (04) : 345 - 354
  • [2] CD4+FoxP3+ regulatory T cells confer infectious tolerance in a TGF-β-dependent manner
    Andersson, John
    Tran, Dat Q.
    Pesu, Marko
    Davidson, Todd S.
    Ramsey, Heather
    O'Shea, John J.
    Shevach, Ethan M.
    [J]. JOURNAL OF EXPERIMENTAL MEDICINE, 2008, 205 (09) : 1975 - 1981
  • [3] Ashraf S, 1998, Transpl Int, V11 Suppl 1, pS5, DOI 10.1007/s001470050415
  • [4] Presence of FoxP3+ regulatory T cells predicts outcome of subclinical rejection of renal allografts
    Bestard, Oriol
    Cruzado, Josep M.
    Rama, Ines
    Torras, Joan
    Goma-i-Freixanet, Montse
    Seron, Daniel
    Moreso, Francesc
    Gil-Vernet, Salvador
    Grinyo, Josep M.
    [J]. JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, 2008, 19 (10): : 2020 - 2026
  • [5] Natural versus adaptive regulatory T cells
    Bluestone, JA
    Abbas, AK
    [J]. NATURE REVIEWS IMMUNOLOGY, 2003, 3 (03) : 253 - 257
  • [6] FOXP3 expression in human kidney transplant biopsies is associated with rejection and time post transplant but not with favorable outcomes
    Bunnag, S.
    Allanach, K.
    Jhangri, G. S.
    Sis, B.
    Einecke, G.
    Mengel, M.
    Mueller, T. F.
    Halloran, P. F.
    [J]. AMERICAN JOURNAL OF TRANSPLANTATION, 2008, 8 (07) : 1423 - 1433
  • [7] Low circulating regulatory T-cell levels after acute rejection in liver transplantation
    Demirkiran, A
    Kok, A
    Kwekkeboom, J
    Kusters, JG
    Metselaar, HJ
    Tilanus, HW
    van der Laan, LJW
    [J]. LIVER TRANSPLANTATION, 2006, 12 (02) : 277 - 284
  • [8] Intragraft FOXP3 mRNA expression reflects antidonor immune reactivity in cardiac allograft patients
    Dijke, I. Esme
    Velthuis, Jurien H. L.
    Caliskan, Kadir
    Korevaar, Sander S.
    Maat, Alex P. W. M.
    Zondervan, Pieter E.
    Balk, Aggie H. M. M.
    Weimar, Willem
    Baan, Carla C.
    [J]. TRANSPLANTATION, 2007, 83 (11) : 1477 - 1484
  • [9] Foxp3 Programs the Development and Function of CD4+CD25+ Regulatory T Cells (Reprinted from vol 4, pg 330-336, 2003)
    Fontenot, Jason D.
    Gavin, Marc A.
    Rudensky, Alexander Y.
    [J]. JOURNAL OF IMMUNOLOGY, 2017, 198 (03) : 986 - 992
  • [10] The regulatory/cytotoxic graft-infiltrating T cells differentiate renal allograft borderline change from acute rejection
    Grimbert, Philippe
    Mansour, Hicham
    Desvaux, D.
    Roudot-Thoraval, Francoise
    Audard, Vincent
    Dahan, Karine
    Berrehar, Francois
    Dehoulle-Poillet, Catherine
    Farcet, Jean Pierre
    Lang, Philippe
    Le Gouvello, Sabine
    [J]. TRANSPLANTATION, 2007, 83 (03) : 341 - 346
123