Open source fraction collector/MALDI spotter for proteomics

被引：10
作者：
Ficarro, Scott B. [1 ,2 ]
Alexander, William Max [1 ,2 ]
Tavares, Isidoro [1 ,2 ]
Marto, Jarrod A. [1 ,2 ]
机构：
[1] Brigham & Womens Hosp, Dana Farber Canc Inst, Blais Prote Ctr, Dept Canc Biol,Dept Pathol, 360 Longwood Ave, LC 2208, Boston, MA 02215 USA
[2] Harvard Med Sch, 360 Longwood Ave,LC 2208, Boston, MA 02215 USA
来源：
HARDWAREX | 2022年 / 11卷
关键词：
Open source; Fraction collection; LC-MALDI; !text type='Python']Python[!/text; Proteomics; MULTIDIMENSIONAL LIQUID-CHROMATOGRAPHY;
D O I：
10.1016/j.ohx.2022.e00305
中图分类号：
TM [电工技术]; TN [电子技术、通信技术];
学科分类号：
0808 ; 0809 ;
摘要：
We describe a complete open-source hardware/software solution for high performance thermostatted peptide fraction collection to support mass spectrometry experiments with complex proteomes. The instrument is easy to assemble using parts readily available through retail channels at a fraction of the cost compared to typical commercial systems. Control software is written in Python allowing for rapid customization. We demonstrate several useful applications, including the automated deposition of LC separated peptides for matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) as well as collection and concatenation of peptide fractions from nanoflow HPLC separations.(c) 2022 The Author(s). Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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页数：56
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  • [1] Low-cost, sub-micron resolution, wide-field computational microscopy using opensource hardware
    Aidukas, Tomas
    Eckert, Regina
    Harvey, Andrew R.
    Waller, Laura
    Konda, Pavan C.
    [J]. SCIENTIFIC REPORTS, 2019, 9 (1)
  • [2] multiplierz v2.0: A Python']Python-based ecosystem for shared access and analysis of native mass spectrometry data
    Alexander, William M.
    Ficarro, Scott B.
    Adelmant, Guillaume
    Marto, Jarrod A.
    [J]. PROTEOMICS, 2017, 17 (15-16)
  • [3] Recent advances in robotic protein sample preparation for clinical analysis and other biomedical applications
    Alexovic, Michal
    Urban, Pawel L.
    Tabani, Hadi
    Sabo, Jan
    [J]. CLINICA CHIMICA ACTA, 2020, 507 : 104 - 116
  • [4] Facile analysis of metabolites by capillary electrophoresis coupled to matrix-assisted laser desorption/ionization mass spectrometry using target plates with polysilazane nanocoating and grooves
    Amantonico, Andrea
    Urban, Pawel L.
    Zenobi, Renato
    [J]. ANALYST, 2009, 134 (08) : 1536 - 1540
  • [5] Off-Line High-pH Reversed-Phase Fractionation for In-Depth Phosphoproteomics
    Batth, Tanveer S.
    Francavilla, Chiara
    Olsen, Jesper V.
    [J]. JOURNAL OF PROTEOME RESEARCH, 2014, 13 (12) : 6176 - 6186
  • [6] Low-cost, scalable, and automated fluid sampling for fluidics applications
    Booeshaghi, A. Sina
    Kil, Yeokyoung
    Min, Kyung Hoi
    Gehring, Jase
    Pachter, Lior
    [J]. HARDWAREX, 2021, 10
  • [7] MEF2C Phosphorylation Is Required for Chemotherapy Resistance in Acute Myeloid Leukemia
    Brown, Fiona C.
    Still, Eric
    Koche, Richard P.
    Yim, Christina Y.
    Takao, Sumiko
    Cifani, Paolo
    Reed, Casie
    Gunasekera, Shehana
    Ficarro, Scott B.
    Romanienko, Peter
    Mark, Willie
    McCarthy, Craig
    de Stanchina, Elisa
    Gonen, Mithat
    Seshan, Venkatraman
    Bhola, Patrick
    O'donnell, Conor
    Spitzer, Barbara
    Stutzke, Crystal
    Lavallee, Vincent-Philippe
    Hebert, Josee
    Krivtsov, Andrei, V
    Melnick, Ari
    Paietta, Elisabeth M.
    Tallman, Martin S.
    Letai, Anthony
    Sauvageau, Guy
    Pouliot, Gayle
    Levine, Ross
    Marto, Jarrod A.
    Armstrong, Scott A.
    Kentsis, Alex
    [J]. CANCER DISCOVERY, 2018, 8 (04) : 478 - 497
  • [8] LEGO MINDSTORMS Fraction Collector: A Low-Cost Tool for a Preparative High-Performance Liquid Chromatography System
    Caputo, Marco
    Lyles, James T.
    Salazar, Monique S.
    Quave, Cassandra L.
    [J]. ANALYTICAL CHEMISTRY, 2020, 92 (02) : 1687 - 1690
  • [9] Recent advances in peptide separation by multidimensional liquid chromatography for proteome analysis
    Di Palma, Serena
    Hennrich, Marco L.
    Heck, Albert J. R.
    Mohammed, Shabaz
    [J]. JOURNAL OF PROTEOMICS, 2012, 75 (13) : 3791 - 3813
  • [10] Review of Three-Dimensional Liquid Chromatography Platforms for Bottom-Up Proteomics
    Duong, Van-An
    Park, Jong-Moon
    Lee, Hookeun
    [J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2020, 21 (04)
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