The Isolation of a Pseudomonas aeruginosa Strain and the Effects on the Degradation of Malachite Green and its Enzymatic Mechanism

被引：1

作者：

Wang, Zhongquan ^{[1
]}

Wen, Yanmao ^{[1
]}

机构：

[1] Univ Elect Sci & Technol China, Zhongshan Inst, Zhongshan 528403, Guangdong, Peoples R China

来源：

CIVIL, STRUCTURAL AND ENVIRONMENTAL ENGINEERING, PTS 1-4 | 2014年 / 838-841卷

关键词：

Malachite green; degradation; enzymatic mechanism; Pseudomonas aeruginosa; CRYSTAL VIOLET; CRUDE-OIL; DECOLORIZATION; BIODEGRADATION; DYES; TRIPHENYLMETHANE; BIOSURFACTANTS; CLONING; GENE;

D O I：

10.4028/www.scientific.net/AMR.838-841.2745

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

A strain isolated from activative sludge, on microbial degradation of malachite green and its enzymatic mechanism was studied. The isolation of microorganism, malachite green degradation and enzyme activity assay experiment, was determined the effects of microbial degradation of malachite green and its mechanism. The 16SrDNA gene sequence of a malachite degrading bacterial isolated was 99.8% similar to Pseudomonas aeruginosa AS2 and identified as Pseudomonas aeruginosa. The P aeruginosa strain could degrade malachite green in the 4000 mg.L-1, and its degrading volcity was positively related to PPO and CMCase activities. The isolation has ability on degrading of high concentration of malachite green.

引用

页码：2745 / +

页数：3

共 33 条

[1] Enhanced biodegradation of Casablanca crude oil by a microbial consortium in presence of a rhamnolipid produced by Pseudomonas aeruginosa AT10 [J].

Abalos, A ;

Viñas, M ;

Sabaté, J ;

Manresa, MA ;

Solanas, AM .

BIODEGRADATION, 2004, 15 (04) :249-260

[2] Biodegradation and decolorization of triphenylmethane dyes by Staphylococcus epidermidis [J].

Ayed, Lamia ;

Chaieb, Kamel ;

Cheref, Abdelkarim ;

Bakhrouf, Amina .

DESALINATION, 2010, 260 (1-3) :137-146

[3] Biodegradation of triphenylmethane dye Malachite Green by Sphingomonas paucimobilis [J].

Ayed, Lamia ;

Chaieb, Kamel ;

Cheref, Abdelkarim ;

Bakhrouf, Amina .

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2009, 25 (04) :705-711

[4] Role of rhamnolipid biosurfactants in the uptake and mineralization of hexadecane in Pseudomonas aeruginosa [J].

Beal, R ;

Betts, WB .

JOURNAL OF APPLIED MICROBIOLOGY, 2000, 89 (01) :158-168

[5] Enhancement of phenanthrene solubilization and biodegradation by trehalose lipid biosurfactants [J].

Chang, JS ;

Radosevich, M ;

Jin, Y ;

Cha, DK .

ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, 2004, 23 (12) :2816-2822

[6] Partial degradation mechanisms of malachite green and methyl violet B by Shewanella decolorationis NTOU1 under anaerobic conditions [J].

Chen, C. -H. ;

Chang, C. -F. ;

Liu, S. -M. .

JOURNAL OF HAZARDOUS MATERIALS, 2010, 177 (1-3) :281-289

[7] Cloning and characterization of a chromosome-encoded catechol 2,3-dioxygenase gene from Pseudomonas aeruginosa ZD 4-3 [J].

Chen, YX ;

Liu, H ;

Zhu, LC ;

Jin, YF .

MICROBIOLOGY, 2004, 73 (06) :689-695

[8] Triphenylmethanes, malachite green and crystal violet dyes decolourisation by Sphingamonas paucimobilis [J].

Cheriaa, Jihane ;

Bakhrouf, Amina .

ANNALS OF MICROBIOLOGY, 2009, 59 (01) :57-61

[9] Biodegradation of malachite green by Pseudomonas sp. strain DY1 under aerobic condition: characteristics, degradation products, enzyme analysis and phytotoxicity [J].

Du, Lin-Na ;

Wang, Sheng ;

Li, Gang ;

Wang, Bing ;

Jia, Xiao-Ming ;

Zhao, Yu-Hua ;

Chen, Yun-Long .

ECOTOXICOLOGY, 2011, 20 (02) :438-446

[10]

Gennaro P D, 2010, APPL MICROBIOL BIOT, V87, P297

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