Role of the Mycobacterium marinum ESX-1 Secretion System in Sliding Motility and Biofilm Formation

被引：24

作者：

Lai, Li-Yin ^{[1
]}

Lin, Tzu-Lung ^{[1
]}

Chen, Yi-Yin ^{[2
]}

Hsieh, Pei-Fang ^{[1
]}

Wang, Jin-Town ^{[1
,3
]}

机构：

[1] Natl Taiwan Univ, Dept Microbiol, Coll Med, Taipei, Taiwan

[2] Chang Gung Univ, Coll Med, Chang Gung Mem Hosp, Dept Pediat,Chang Gung Childrens Hosp, Taoyuan, Taiwan

[3] Natl Taiwan Univ Hosp, Dept Internal Med, Taipei, Taiwan

来源：

FRONTIERS IN MICROBIOLOGY | 2018年 / 9卷

关键词：

type VII secretion system; Mycobacterium marinum; ESX-1; sliding motility; biofilm formation; VII SECRETION; DICTYOSTELIUM-DISCOIDEUM; VIRULENCE FACTOR; GENE-CLUSTER; BIOSYNTHESIS; PROTEIN; TUBERCULOSIS; GLYCOPEPTIDOLIPIDS; IDENTIFICATION; TRANSFORMATION;

D O I：

10.3389/fmicb.2018.01160

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Mycobacterium marinum is a close relative of Mycobacterium tuberculosis that can cause systemic tuberculosis-like infections in ectotherms and skin infections in humans. Sliding motility correlates with biofilm formation and virulence in most bacteria. In this study, we used a sliding motility assay to screen 2,304 transposon mutants of M. marinum NTUH-M6885 and identified five transposon mutants with decreased sliding motility. Transposons that interrupted the type VII secretion system (T7SS) ESX-1-related genes, espE (mmar_5439), espF (mmar_5440), and eccA1 (mmar_5443), were present in 3 mutants. We performed reverse-transcription polymerase chain reaction to verify genes from mmar_5438 to mmar_5450, which were found to belong to a single transcriptional unit. Deletion mutants of espE, espF, espG (mmar_5441), and espH (mmar_5442) displayed significant attenuation regarding sliding motility and biofilm formation. M. marinum NTUH-M6885 possesses a functional ESX-1 secretion system. However, deletion of espG or espH resulted in slightly decreased secretion of EsxB (which is also known as CFP-10). Thus, the M. marinum ESX-1 secretion system mediates sliding motility and is crucial for biofilm formation. These data provide new insight into M. marinum biofilm formation.

引用

页数：12

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