Genome-Wide Approach in Arabidopsis thaliana to Assess the Toxicity of Cadmium Sulfide Quantum Dots

被引:63
作者
Marmiroli, M. [1 ]
Pagano, L. [1 ]
Sardaro, M. L. Savo [1 ]
Villani, M. [2 ]
Marmiroli, N. [1 ]
机构
[1] Univ Parma, Dept Life Sci, I-43100 Parma, Italy
[2] CNR, IMEM, I-43214 Parma, Italy
关键词
NANOPARTICLES; ZNO; ECOTOXICOLOGY; PHYTOTOXICITY; CHEMISTRY; BIOLOGY; PLANTS; GENES; CEO2;
D O I
10.1021/es404958r
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Cadmium sulfide quantum dots (CdS QDs) are used in the manufacture of a number of electronics products. Their small size allows their ready entry into living cells, but as yet no attempt has been made to assess their toxicity. Our aim was to exploit two Ds transposition-induced mutant lines of Arabidopsis thaliana which tolerated exposure to CdS QDs to identify the genetic basis of their tolerance. Both a genome-wide top-down (from mutant to genes) and a bottom-up (from gene expression to phenotype) approach were applied. The differential responses of the mutants compared to the wild type showed that sensitivity to CdS QDs was unrelated to sensitivity to Cd2+ ions. A transcriptomic analysis identified a number of genes whose transcript abundance was correlated with the tolerance. The phenotype of one of the mutants was correlated with the overexpression of ELM2, an MYB containing gene visited by a Ds transposon. Segregation analysis showed that the genetic basis of CdS QDs tolerance in both mutants was monogenic. The phenotype of the other mutant could be explained by the mutation of HCF101, a gene involved in photosynthesis.
引用
收藏
页码:5902 / 5909
页数:8
相关论文
共 39 条
  • [1] DEVELOPMENT OF AN EFFICIENT 2-ELEMENT TRANSPOSON TAGGING SYSTEM IN ARABIDOPSIS-THALIANA
    BANCROFT, I
    BHATT, AM
    SJODIN, C
    SCOFIELD, S
    JONES, JDG
    DEAN, C
    [J]. MOLECULAR AND GENERAL GENETICS, 1992, 233 (03): : 449 - 461
  • [2] BCC Research, 2011, NANOTECHNOLOGY A REA
  • [3] Nanomaterials and nanoparticles: Sources and toxicity
    Buzea, Cristina
    Pacheco, Ivan I.
    Robbie, Kevin
    [J]. BIOINTERPHASES, 2007, 2 (04) : MR17 - MR71
  • [4] The MYB transcription factor superfamily of arabidopsis: Expression analysis and phylogenetic comparison with the rice MYB family
    Chen, YH
    Yang, XY
    He, K
    Liu, MH
    Li, JG
    Gao, ZF
    Lin, ZQ
    Zhang, YF
    Wang, XX
    Qiu, XM
    Shen, YP
    Zhang, L
    Deng, XH
    Luo, JC
    Deng, XW
    Chen, ZL
    Gu, HY
    Qu, LJ
    [J]. PLANT MOLECULAR BIOLOGY, 2006, 60 (01) : 107 - 124
  • [6] MYB transcription factors in Arabidopsis
    Dubos, Christian
    Stracke, Ralf
    Grotewold, Erich
    Weisshaar, Bernd
    Martin, Cathie
    Lepiniec, Loic
    [J]. TRENDS IN PLANT SCIENCE, 2010, 15 (10) : 573 - 581
  • [7] Cluster analysis and display of genome-wide expression patterns
    Eisen, MB
    Spellman, PT
    Brown, PO
    Botstein, D
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (25) : 14863 - 14868
  • [8] Surface properties of CdS nanoparticles
    Favero, P. P.
    de Souza-Parise, M.
    Fernandez, J. L. R.
    Miotto, R.
    Ferraz, A. C.
    [J]. BRAZILIAN JOURNAL OF PHYSICS, 2006, 36 (3B) : 1032 - 1034
  • [9] T-DNA INSERTION MUTAGENESIS IN ARABIDOPSIS - MUTATIONAL SPECTRUM
    FELDMANN, KA
    [J]. PLANT JOURNAL, 1991, 1 (01) : 71 - 82
  • [10] Phytotoxicity, accumulation and transport of silver nanoparticles by Arabidopsis thaliana
    Geisler-Lee, Jane
    Wang, Qiang
    Yao, Ying
    Zhang, Wen
    Geisler, Matt
    Li, Kungang
    Huang, Ying
    Chen, Yongsheng
    Kolmakov, Andrei
    Ma, Xingmao
    [J]. NANOTOXICOLOGY, 2013, 7 (03) : 323 - 337