Oestrogenic regulation and differential expression of WNT4 in the bonnet monkey and rodent epididymis

被引：9

作者：

Deshpande, Shayu N. ^{[2
]}

Vijayakumar, Govindaraj ^{[3
]}

Rao, A. Jagannadha ^{[1
,4
,5
]}

机构：

[1] Indian Inst Sci, Dept Biochem, Bangalore 560012, Karnataka, India

[2] Mem Sloan Kettering Canc Res Ctr, Dept Dev Biol, New York, NY 10021 USA

[3] Univ Wurzburg, Dept Mol Cardiol, D-97080 Wurzburg, Germany

[4] Univ Calif San Francisco, San Francisco, CA 94143 USA

[5] Univ Sherbrooke, Sherbrooke, PQ J1K 2R1, Canada

来源：

REPRODUCTIVE BIOMEDICINE ONLINE | 2009年 / 18卷 / 04期

基金：

美国安德鲁·梅隆基金会;

关键词：

epididymis; monkey; oestrogen; WNT4; RECEPTOR; TESTIS; RAT;

D O I：

10.1016/S1472-6483(10)60134-4

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

A role for oestrogen in regulating fluid reabsorption in the monkey epididymis was recently demonstrated. Here, these Studies are extended to identify potential oestrogen-regulated proteins in the cauda region of monkey epididymis treated with vehicle and oestrogen receptor antagonist (ICI 182780). Two-dimensional electrophoretic analysis was used to identify the proteins. The results indicated down-regulation of WNT4 in the ICI-182780-treated monkey cauda. In addition. the Wnt4f mRNA concentration was also reduced in the caput regions of ICI-182780-treated rats and oestrogen receptor knockout mice. WNT4 is a key regulator of gonadal differentiation in humans and mice and plays a pivotal role in early mouse embryogenesis. The results of the present Study establish the presence of WNT4 in the monkey epididymis and its regulation by oestrogen, and Suggest a role for WNT4 in maintaining epididymal homeostasis.

引用

页码：555 / 561

页数：7

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