Inhibition of TNF-α-Mediated NF-κB Transcriptional Activity by Dammarane-Type Ginsenosides from Steamed Flower Buds of Panax ginseng in HepG2 and SK-Hep 1 Cells

被引：18
作者：
Cho, Kyoungwon [1 ]
Song, Seok Bean [1 ]
Nguyen Huu Tung [1 ]
Kim, Kyoon Eon [2 ]
Kim, Young Ho [1 ]
机构：
[1] Chungnam Natl Univ, Coll Pharm, Taejon 305764, South Korea
[2] Chungnam Natl Univ, Dept Biochem, Taejon 305764, South Korea
来源：
BIOMOLECULES & THERAPEUTICS | 2014年 / 22卷 / 01期
基金：
新加坡国家研究基金会;
关键词：
NF-kappa B inhibitory activity; Panax ginseng flower buds; Tumor necrosis factor-alpha; Hepatocyte derived cells; LIVER-CANCER; INFLAMMATION; SAPONINS; LEAVES; TARGET;
D O I：
10.4062/biomolther.2013.096
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Panax ginseng is a medicinal herb that is used worldwide. Its medicinal effects are primarily attributable to ginsenosides located in the root, leaf, seed, and flower. The flower buds of Panax ginseng (FBPG) are rich in various bioactive ginsenosides, which exert immunomodulatory and anti-inflammatory activities. The aim of the present study was to assess the effect of 18 ginsenosides isolated from steamed FBPG on the transcriptional activity of NF-kappa B and the expression of tumor necrosis factor-alpha (INF-alpha)-stimulated target genes in liver-derived cell lines. Noticeably, the ginsenosides Rk(3) and Rs(4) exerted the strongest activity, inhibiting NF-kappa B in a dose-dependent manner. SF and Rg(6) also showed moderately inhibitory effects. Furthermore, these four compounds inhibited the TNF-alpha-induced expression of 18, CXCL1, iNOS, and ICAM1 genes. Consequently, ginsenosides purified from steamed FBPG have therapeutic potential in TNF-alpha-mediated diseases such as chronic hepatic inflammation.
引用
收藏
页码：55 / 61
页数：7
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