Everything you ever wanted to know about PKA regulation and its involvement in mammalian sperm capacitation

被引：39

作者：

Baro Graf, Carolina ^{[1
,2
]}

Ritagliati, Carla ^{[1
]}

Stival, Cintia ^{[1
]}

Luque, Guillermina M. ^{[3
]}

Gentile, Inaki ^{[1
]}

Buffone, Mariano G. ^{[3
]}

Krapf, Dario ^{[1
,2
]}

机构：

[1] CONICET UNR, Lab Cell Signal Transduct Networks, Inst Biol Mol & Celular Rosario IBR, Rosario, Argentina

[2] Univ Nacl Rosario, Fac Ciencias Bioquim & Farrnaceut, Lab Med Reprod LMR, Rosario, Argentina

[3] Consejo Nacl Invest Cient & Tecn, Lab Cellular & Mol Reprod Biol, Inst Biol & Med Expt IBYME, Buenos Aires, DF, Argentina

来源：

MOLECULAR AND CELLULAR ENDOCRINOLOGY | 2020年 / 518卷

关键词：

Protein kinase A (PKA); Sperm capacitation; Reproduction; cAMP; PROTEIN-KINASE-A; SOLUBLE ADENYLYL-CYCLASE; EPSILON-N-ACETYLLYSINE; BOVINE CARDIAC-MUSCLE; CATALYTIC SUBUNIT; MOUSE SPERM; TYROSINE PHOSPHORYLATION; ANCHORING PROTEIN; FIBROUS SHEATH; LYSINE ACETYLATION;

D O I：

10.1016/j.mce.2020.110992

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The 3', 5'-cyclic adenosine monophosphate (cAMP) dependent protein kinase (PKA) is a tetrameric holoenzyme comprising a set of two regulatory subunits (PKA-R) and two catalytic (PKA-C) subunits. The PKA-R subunits act as sensors of cAMP and allow PKA-C activity. One of the first signaling events observed during mammalian sperm capacitation is PKA activation. Thus, understanding how PKA activity is restricted in space and time is crucial to decipher the critical steps of sperm capacitation. It is widely accepted that PKA specificity depends on several levels of regulation. Anchoring proteins play a pivotal role in achieving proper localization signaling, subcellular targeting and cAMP microdomains. These multi-factorial regulation steps are necessary for a precise spatiotemporal activation of PKA. Here we discuss recent understanding of regulatory mechanisms of PKA in mammalian sperm, such as post-translational modifications, in the context of its role as the master orchestrator of molecular events conducive to capacitation.

引用

页数：18

共 275 条

[81] Engineering of a red-light-activated human cAMP/cGMP-specific phosphodiesterase [J].