Non-invasive Chamber-Specific Identification of Cardiomyocytes in Differentiating Pluripotent Stem Cells

被引：32

作者：

Brauchle, Eva ^{[1
,2
]}

Knopf, Anne ^{[1
,2
,3
]}

Bauer, Hannah ^{[2
]}

Shen, Nian ^{[1
,2
]}

Linder, Sandra ^{[1
]}

Monaghan, Michael G. ^{[1
,2
]}

Ellwanger, Kornelia ^{[4
]}

Layland, Shannon L. ^{[1
,2
]}

Brucker, Sara Y. ^{[2
]}

Nsair, Ali ^{[3
]}

Schenke-Layland, Katja ^{[1
,2
,3
]}

机构：

[1] Fraunhofer Inst Interfacial Engn & Biotechnol IGB, Dept Cell & Tissue Engn, Nobelstr 12, D-70569 Stuttgart, Germany

[2] Univ Tubingen, Res Inst Womens Hlth, Dept Womens Hlth, Silcherstr 7-1, D-72076 Tubingen, Germany

[3] Univ Calif Los Angeles, David Geffen Sch Med, Cardiovasc Res Labs, Dept Med Cardiol, 675 Charles E Young Dr South,MRL 3645, Los Angeles, CA 90095 USA

[4] Univ Stuttgart, Inst Cell Biol & Immunol, Allmandring 31, D-70569 Stuttgart, Germany

来源：

STEM CELL REPORTS | 2016年 / 6卷 / 02期

关键词：

RAMAN-SPECTROSCOPY; MOUSE; MATURATION; PROTEINS; STAGE;

D O I：

10.1016/j.stemcr.2015.12.007

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

One major obstacle to the application of stem cell-derived cardiomyocytes (CMs) for disease modeling and clinical therapies is the inability to identify the developmental stage of these cells without the need for genetic manipulation or utilization of exogenous markers. In this study, we demonstrate that Raman microspectroscopy can non-invasively identify embryonic stem cell (ESC)-derived chamber-specific CMs and monitor cell maturation. Using this marker-free approach, Raman peaks were identified for atrial and ventricular CMs, ESCs were successfully discriminated from their cardiac derivatives, a distinct phenotypic spectrum for ESC-derived CMs was confirmed, and unique spectral differences between fetal versus adult CMs were detected. The real-time identification and characterization of CMs, their progenitors, and subpopulations by Raman microspectroscopy strongly correlated to the phenotypical features of these cells. Due to its high molecular resolution, Raman microspectroscopy offers distinct analytical characterization for differentiating cardiovascular cell populations.

引用

页码：188 / 199

页数：12

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