Molecular characterization and bio-functional property determination using SDS-PAGE and RP-HPLC of protein fractions from two Nigella species

被引：21

作者：

Alu'datt, Muhammad H. ^{[1
]}

Rababah, Taha ^{[1
]}

Alhamad, Mohammad N. ^{[2
]}

Alodat, Moh'd ^{[3
]}

Al-Mahasneh, Majdi A. ^{[4
]}

Gammoh, Sana ^{[1
]}

Ereifej, Khalil ^{[1
]}

Almajwal, Ali ^{[5
]}

Kubow, Stan ^{[6
]}

机构：

[1] Jordan Univ Sci & Technol, Fac Agr, Dept Nutr & Food Technol, POB 3030, Irbid 22110, Jordan

[2] Jordan Univ Sci & Technol, Dept Nat Resources & Environm, Fac Agr, POB 3030, Irbid 22110, Jordan

[3] Yarmouk Univ, Dept Stat, Fac Sci, Irbid, Jordan

[4] Jordan Univ Sci & Technol, Dept Chem Engn, POB 3030, Irbid 22110, Jordan

[5] King Saud Univ, Dept Community Hlth Sci, Coll Appl Med Sci, POB 10219, Riyadh 11433, Saudi Arabia

[6] McGill Univ, Sch Dietet & Human Nutr, Montreal, PQ, Canada

来源：

FOOD CHEMISTRY | 2017年 / 230卷

关键词：

Nigella species; RP-HPLC fractionation; Protein fractions; Molecular; Angiotensin converting enzyme; Fractionation; Antioxidant activity; I-CONVERTING-ENZYME; SATIVA L SEEDS; ANTIOXIDANT ACTIVITY; CHEMICAL-COMPOSITION; BIOACTIVE PEPTIDES; STORAGE PROTEINS; CHRONIC DISEASES; POTENTIAL ROLE; IDENTIFICATION; PREVENTION;

D O I：

10.1016/j.foodchem.2017.03.025

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

This study aimed to investigate the molecular and bio-functional properties of protein fractions from Nigella damascena and Nigella arvensis, including the albumin, globulin, glutein-1, glutein-2 and prolamin fractions. Protein subunits were not observed in globulin and prolamin fractions. No peaks appeared in RP-HPLC chromatograms of globulin for either species. Two predominant peaks were observed in the RP-HPLC profiles of all protein fractions. Proteins separated by RP-HPLC have potential inhibitory and antioxidant activities in all fractions. Optimum ACE-inhibitory and antioxidant activities of proteins separated by RP-HPLC were observed in glutein-2 and albumin, respectively, for both species. For pepsin and combined pepsin-trypsin hydrolyses, the highest degree of hydrolysis (DH) was obtained in glutein-2 fraction of Nigella arvensis. Highest ACE-inhibitory activity of hydrolyzed protein fractions was found at 4 h via pepsin hydrolysis in globulin fraction of Nigella damascena. Highest antioxidant activities of hydrolyzed protein fractions were found in glutelin-2 for both species. (C) 2017 Elsevier Ltd. All rights reserved.

引用

页码：125 / 134

页数：10

共 39 条

[1]

ABDELAAL ESM, 1993, ALEXANDRIA SCI EXCHA, V14, P483

[2]

Ahn J. Y., 2001, THESIS

[3] The anti-inflammatory, analgesic and antipyretic activity of Nigella sativa [J].